急性髓细胞白血病端粒酶活性及临床意义

Telomerase activity in acute myeloid leukemia and its clinical significance

目的 :探讨端粒酶活性在急性髓细胞白血病 ( AML )发生中的作用。方法 :采用端粒重复序列扩增文件酶标法 ( TRAP- EL ISA) ,对 32例 AML 和非恶性血液系统疾病骨髓细胞端粒酶活性检测。结果 :端粒酶活性在初发 AML 患者高于非恶性血液系统疾病患者 ( P <0 .0 1) ,AML 初发期患者高于完全缓解期患者 ( P <0 .0 5 ) ,端粒酶活性与体内白血病细胞数之间无显著相关性。结论

Objective:To evaluate the role of telomerase activity in development of acute myeloid leukemia.Methods:The telomerase activity was determined in bone marrow cells from 32 patients of acute myeloid leukemia and non malignant hematogic disease by TRAP ELISA.Results:Telomerase activity is higher in AML than in non malignant hematologic disease (P< 0.01 ) and higher in untreated patients than in complete remissive patient (P< 0.05 ).There is no significant correlation between telomerase activity and total leukemia cells in patient.Conclusion:Detection of telomerase activity might help to predict the thrapeutic effect of AML patients.