雷洛昔芬对大鼠垂体瘤GH3细胞Seladin-1基因表达与增殖的影响

Effects of raloxifene on proliferation and seladin-1 gene expression in GH3 cells

目的探讨选择性雌激素受体拮抗剂雷洛昔芬对大鼠垂体瘤GH3细胞株的seladin-1基因表达及其对瘤细胞增殖的影响。方法以不同浓度(0.1～1 000 nmol/L)的雷洛昔芬作用于大鼠GH3细胞,培养48 h后检测雷洛昔芬的作用效果;荧光定量RT-PCR和Western blot分别检测seladin-1mRNA和蛋白;Cell Counting Kit-8试剂盒检测不同浓度药物对瘤细胞增殖的影响。结果雷洛昔芬在10～100 nmol/L的浓度范围能有效抑制selandin-1的表达,瘤细胞增殖受抑制(P<0.05)。结论雷洛昔芬能够有效抑制大鼠垂体瘤GH3细胞株selandin-1表达及瘤细胞生长,可作为垂体瘤的药物和基因治疗的新靶点。

Objective To explore the effects of raloxifene,a selective estrogen receptor antagonist,on proliferation and seladin-1 gene expression in rat GH-secreting（GH3）cells.Methods 48 h after raloxifene was added in a series of concentrations（0.1~1 000 nmol/L）to GH3 cells,the expression level of the seladin-1 gene was examined by quantitative real time RT-PCR and Western blot,and cell proliferation was investigated using the Cell Counting Kit-8.Results With quantitative real time RT-PCR and Western blot,it was found that,with the drug within the 10-100 nmol/L range,seladin-1 expression was significantly down-regulated and cell proliferation was inhibited in a concentration-dependent manner（P0.05）.Conclusion Raloxifene suppresses seladin-1 expression and GH3 cell proliferation and,hence,it holds the promise of being used in treating pituitary adenoma.The seladin-1 gene may be a new target for gene therapy.