短串联重复序列基因座与冲动暴力行为的关联研究

Association study of the genetic polymorphism of fifteen short tandem repeats loci and aggressive violent behavior

目的探讨冲动暴力行为与相关短串联重复序列基因座的关联情况。方法运用AmpFlSTRIdentifiler^TM荧光标记复合扩增体系，对203例冲动暴力行为罪犯（研究组）与207名非暴力行为健康个体（对照组）样本进行聚合酶链反应复合扩增，然后应用ABl3100型基因分析系统对扩增产物进行电泳和基因检测，观察2组15个STR基因座等位基因及基因型频率的差异。结果15个STR基因座均符合遗传平衡定律（Hardy—Weinberg定律）；研究组与对照组TH01和TPOX基因座的等位基因频率分布的差异有统计学意义（P〈0．05）；研究组和对照组TH01—10频率分别为0．0172和0．0580，差异有统计学意义（P=0．002，OR=0．29，95％CI：0．12～0．67）；研究组和对照组TPOX-11频率分别为0．3621和0．2391，差异有统计学意义（P=0．000，OR=1．81，95％CI：1．33～2．45）；其他STR基因座等位基因频率及所有基因型频率2组差异均无统计学意义（P〉0．05）。结论TH01和TPOX基因座多态性与冲动暴力行为可能存在关联，等位基因TH01-10、TPOX—11与冲动暴力行为的发生可能有一定关系。

Objective To investigate the association of aggressive violent behavior and related short tandem repeats （STRs） loci by the analysis of 15 STRs loci genetic polymorphism. Methods The biological samples of 203 persons with aggressive violent behaviors and 207 healthy persons without violent behavior were collected. Then the DNA sample was amplified by AmpF1STR IdentifilerTM system and separated by electrophoresis to compare the genotypes and alleles of 15 STRs gene frequencies in the two groups. Results All the 15 STRs loci in people with aggressive violent behavior and healthy people were found to coincide with Hardy-Weinberg equilibrium （ P 〉 0.05 ）. There was a significant difference in distributing of allele frequency of THO1 and TPOX between people with aggressive violent behaviors and healthy people （ P 〈 0. 05 ）. The frequency of allele 10 of TH01 （ aggressive violent offenders ：0. 0172 ; control group ：0. 0580 ） was significantly different in the two groups （ P = 0. 002, OR = 0. 29,95% CI： O. 12 - 0. 67 ）. The frequency of allele 11 of TPOX （ people with aggressive violent behaviors： 0. 3621 ; healthy people： 0. 2391 ） was significantly different between the two groups（P = 0. 000, OR = 1.81,95% CI： 1.33 - 2.45 ）. There was not a statistical difference in other STRs loci allele frequencies and all the STRs genotype frequencies between the two groups （ P 〉 0. 05 ）. Conclusion The genetic polymorphism of THO1 and TPOX loci may be associated with the aggressive violent behaviors, and the allele 10 of THO1, allele 11 of TPOX may be the genetic factor to aggressive violent behavior.