摘要
研究了盐度23下青蛤(Cyclina sinensis)鳃、斧足、外套膜和内脏团中Na+/K+-ATPase的活性差异,并以活性高的鳃组织为对象,对青蛤实施了低盐(23→16)和高盐(23→36)突变的胁迫,监测了鳃组织中Na+/K+-ATPase活性的动态变化。结果表明,盐度23下青蛤鳃组织中Na+/K+-ATPase的活性显著高于其他组织;在盐度突降胁迫下,鳃组织中Na+/K+-ATPase的活性于前16 d逐渐升高,第17d起显著升高(p<0.05),第18 d达到峰值,于第20 d回落,但仍高于起始水平;在盐度骤升胁迫下,鳃组织中Na+/K+-ATPase的活性于前13d逐渐降低,第14 d起显著降低(p<0.05),于第15 d达到最低值,继而回升至平稳水平,但仍低于起始水平。青蛤不同盐度胁迫下其鳃组织中Na+/K+-ATPase活性的响应方式不同,低盐时活性增加,而高盐时活性下降;此外,青蛤在盐度胁迫下对自身渗透压的调节则需较长时间。
The difference of Na＋/K＋-ATPase activity among tissues（gill,foot,mantle and viscera） in Cyclina sinensis at the normal salinity 23 was investigated.Afterwards,the dynamic changes of Na＋/K＋-ATPase activity in the resulting tissue which had a high value during the normal salinity was focused and detected when C.sinensis suffered an abrupt decrease（from 23 to 16） and increase（from 23 to 36） in salinity.The results showed that Na＋/K＋-ATPase activity in gills was significantly higher than other three tissues when C.sinensis exposed at normal salinity.After an abrupt decrease in salinity,Na＋/K＋-ATPase activity in gills didn’t present a significant difference in the first 16 days（p0.05）,and it began to increase significantly from the 17th day to the 19th day（p0.05）,and returned to the initial value at the 20th day.While stimulated by an acute elevation of salinity,no significant diversity of Na＋/K＋-ATPase activity was observed in the first 13 days（p0.05）,and it began to decrease at the 14th day,and reached the nadir at the 15th day.Thereafter,it gradually increased to the initial value in the following time.In conclusion,the response way of Na＋/ K＋-ATPase activity in the gills of C.sinensis which was under different salinity stress was different,showing increased activity for the low salinity stress,and decreased activity for the high salinity stress.Additionally,and it took C.sinensis a long time to balance its plasma osmolality after a salinity stress.
出处
《广东海洋大学学报》
CAS
2012年第1期54-58,共5页
Journal of Guangdong Ocean University
基金
上海市科委技术标准专项(10DZ0503100)
