比色法测定保鲜人参中人参总皂苷的含量

Determination of total ginsenoside content in fresh-keeping ginseng by colorimetry

目的采用索氏热回流提取保鲜人参中的人参总皂苷。方法香草醛-硫酸比色法测定保鲜人参中人参总皂苷的含量,检测波长544nm。结果索氏热回流提取可将保鲜人参中的人参总皂苷提取完全,在20～120μg(r=0.9995)范围内线性关系良好,平均回收率为107.86%,RSD=3.49%(n=5)。测得样品中总皂苷含量为3.91%,RSD=1.84%(n=3)。结论该方法操作简便,稳定性和重现性较好,可用于保鲜人参中人参总皂苷的含量测定。

The fresh-keeping ginseng was extracted by soxhlet extraction with hot methanol reflux method, And then,The colorimetry method analysis was employed,The detected wavelength was 544 nm. Results indicated th;tt A good linear relationship was observed in the range of 20-120μg （r=0.9995）and averagerecovery rate was 107.86% with a relative standard deviation of 3.49%（n=5）. The content of total ginsenoside in the samples from Ji＇an city,Ji lin region was 3.91%,with 1.84% of RSD （n=3）.