FCER1A基因单核苷酸多态性与变应性鼻炎血清总IgE含量的相关性分析

Association analysis between single-nucleotide polymorphisms in the FCER1A gene and serum total IgE level in patients with allergic rhinitis

目的探讨FCER1A基因单核苷酸多态性（single—nucleotide polymorphisms，SNP）与中国变应性鼻炎人群血清总IgE含量的相关性。方法以378例确诊的变应性鼻炎患者为研究对象，血清总IgE检测使用UniCAP100检测系统，根据Hapmap网站公布的中国北京地区人群的FCER1A基因标签SNP位点信息及Haploview4．1软件运行结果，选取覆盖FCER1A整个基因的代表性标签SNP位点共8个．MassARRAY实验平台完成各SNP位点的基因型检测，以SPSS13．0软件进行统计学分析。结果378例变应性鼻炎患者的血清总IgE含量在6．94～5000kU／L之间，中位数为150kU／L。Levene检验显示每个位点的不同基因型的患者血清总IgE含量的方差齐，ANOVA检验提示血清总IgE含量与各候选SNP位点基因型无关联性。结论FCER1A基因SNP与本研究的中国北京地区变应性鼻炎患者的血清总IgE含量不相关。

Objective To investigate whether the single nucleotide polymorphisms （SNP） in the FCER1A gene are associated with serum total IgE level in Chinese allergic rhinitis （AR） cohort. Methods A total of 378 patients diagnosed as AR was included in this study. Serum total IgE level was detected by UniCAP100 testing system. A total of 8 reprehensive marker SNP which were in FCER1A gene region were selected according to the Beijing people database from Hapmap website and the running results of Haploview 4. 1 software. The individual genotyping was performed by MassARRAY platform. Results Three hundred and seventy-eight individuals （227 males and 151 females） with AR were prospectively recruited. The median of the total serum IgE measurements for the study population were 150 kU/L and ranged from 6.94 kU/L to 5000 kU/L. Levene tests showed the homogeneity of variance of total IgE level among different genotypes regarding each SNP locus. ANOVA tests demonstrated that none of the selected polymorphism loci in FCER1A was associated with total IgE level. Conclusion Present study failed to find an association between SNP in the FCER1A gene region and serum total IgE level in Chinese AR.