高效液相色谱检测银杏茶中总黄酮含量

Determination of Flavonoids in Ginkgo Biloba Tea by HPLC

采用正交试验法优选银杏茶中黄酮类化合物的最佳提取条件为25%HCl添加量4 mL,提取温度100℃,提取时间90 min。分析方法采用Eclipse XDB C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-0.4%磷酸溶液(体积比60∶40),流速1.0 mL/min,柱温30℃,检测波长为360 nm。槲皮素、山柰酚、异鼠李素在浓度20μg/mL～100μg/mL范围内线性良好,检出限分别为5.0、8.6、6.7 mg/kg。相对标准偏差(RSD)小于5%(n=6)。本法准确、快速、重现性好,可用于银杏茶的质量控制。

The optimum extracting technology of flavonodis in ginkgo biloba tea was investigated by using the factorial and orthogonal design. The optimal conditions included 25 % HCl added 4 mL, extraction temperature100℃, and extraction 1.5 h for each times. The separation was performed on Eclipse XDB C18 column（250 mm×4.6 mm, 5 μm）. The mobile phase was methanol-0.4 % phosphoric acid（60：40, volumn ratio）with a flow rate of 1.0 mL/min. The detection wavelength was set at 360 nm and the column teperature was 30℃. A linear calibration curve of quercetin, isorhamnetin and keampferol was obtained in the concentration range of 20 μg/mL- 100 μg/mL. The qualification limit were 5.0, 8.6, 6.7 mg/kg. The relative standard deviations（RSDs ）were below 5 %（n=6）. The method was simple, accurate and can be used for quality control of ginkgo biloba tea.