摘要
将副溶血弧菌 (Vibrioparahaemolyticus)菌株1211U培养于低温贫养条件下 ,以平板菌落计数法 (PC)和最大近似数法 (MPN)检测可培养细菌数 ,50～60d后表明可培养数下降为零。吖啶橙荧光显微镜直接计数法 (AODC)检测细菌总数 ,表明细菌总数始终变化不大 ,而活菌直接计数法 (DVC)检测到的活菌数保持在105个/ml。本实验证明了副溶血弧菌1211U株在一定的条件下可进入活的非可培养状态 (VBNC)。
Vibrio parahaemolyticus 1211U was cultured in sterilized sea water by the process of filtration at 4 ℃;50 60 days later, plate count(PC) and most probable number(MPN) showed that there were no culturable bacteria. But acridine orange direct count(AODC) showed that the total number of bacteria was not changed appreciably.Direct viable count(DVC) also confirmed that the number of viable Vibrio parahaemolyticus in the water was 105cells/ml.So it is concluded that Vibrio parahaemolyticus 1211U can enter the viable but nonculturable state under certain conditions.
出处
《海洋科学》
CAS
CSCD
北大核心
2000年第6期52-54,28,共4页
Marine Sciences
