实时超声弹性成像评价无水酒精所致兔肝坏死灶的研究

In-vivo investigation of ethanol-induced hepatic lesions using real-time ultrasound elastography

目的探讨应用实时超声弹性成像技术评价无水酒精注射所致兔肝局部坏死灶形态及大小的价值。方法健康日本大耳兔24只,随机分成4组:酒精注射即刻组、1周组、2周组、4周组,每组6只,将1 mL无水酒精注入兔肝左叶,分别用常规二维超声及超声弹性成像测量坏死灶的最大横径,并与大体标本测量的结果进行对照。结果各组弹性图上均显示坏死灶呈均匀蓝色(较硬区),明显区别于周围正常肝组织(绿色区域)。弹性成像测量的各组坏死灶大小与大体标本的相关性(r)分别为0.803、0.854、0.858、0.844(P值分别为0.039、0.030、0.029、0.035)。其中常规二维超声与大体标本测量值比较,差异有统计学意义(P<0.05),实时超声弹性成像与大体标本测量的结果比较差异无统计学意义(P>0.05)。结论常规二维超声不能准确评价坏死灶的大小,而实时超声弹性成像能很好的反映无水酒精所致兔肝坏死灶的大小,实时超声弹性成像可能成为评价无水酒精所致兔肝坏死灶形态及大小的有效方法。

Objective To evaluate the reliability of real-time ultrasound elastography for estimating the ethanol-induced hepatic lesions.Methods In 24 anesthetized rabbits,1 mL of ethanol was injected percutaneously under sonographic guidance.The animals were divided into four groups（each according to the times of gray-scale ultrasound and elastgraphy examinations after PEI）： day 1（immediately after）,week 1,week 2 and week 4.All of the ultrasound and elastgraphy images were stored in computer.Then,the measurements of all the ethanol-induced lesions were done.Results The ethanol-induced lesions could not be correctly evaluated with grey-scale ultrasound.The ethanol-induced lesion boundaries in the elastograms were clearer.The concordance（r） between elastograms and pathologic for the measurements of each group was 0.803,0.854,0.858 and 0.844,respectively（the probability was 0.039,0.030,0.029 and 0.035,respectively）.Conclusion Real-time ultrasound elastography can accurately assess the effect of the ethanol-induced therapy.