美洲大蠊中小分子肽的Tricine-SDS-PAGE凝胶电泳分离探索

Exploration of the Small Peptides Separation by Tricine-SDS-PAGE from Periplaneta americana

[目的]探讨适合美洲大蠊中小分子多肽分离、纯化的Tricine-SDS-PAGE方法。[方法]通过比较Tricine-SDS-PAGE中,不同比例3种胶对小分子肽的分离效果,建立更适合小分子肽分离的方法。[结果]聚丙烯酰胺凝胶电泳在一定程度上使标准Marker得到了初步分离,样品混合液均在分离胶、夹层胶和浓缩胶中有着不同程度的聚集,未能起到分离的作用。[结论]对于小分子多肽的混合样品,有待于进一步摸索更合适的聚丙烯酰胺凝胶电泳分离方法或是其他更好的分离方法。

[Objective] To establish a Tricine-SDS-PAGE method to separate and purify small peptides from Periplaneta americana.[Method] The effects of the Tricine-SDS-PAGE method with different proportions of three gels to separate the small peptides were compared,and the proper method for small peptides separation was established.[Result] The Marker could be separated by Tricine-SDS-PAGE to a certain extent,however,the samples assembled in the separation,spacer and stacking gels and could not been separated.[Conclusion] we should seek for the most appropriate Tricine-SDS-PAGE method or better methods for separating small pepetides.