流式细胞仪在细胞生物学实验教学改革中的应用

Application of Flow Cytometry in the Experimental Teaching Reform of Cytobiology Course

[目的]为本科细胞生物学实验提供新颖选题,提高流式细胞仪利用率。[方法]基于细胞生物学基础知识,应用流式细胞仪,采用不同方法对细胞凋亡进行检测分析。[结果]不同方法的实验结果均可体现和解释细胞凋亡现象,可提高学生对细胞特性和流式细胞术的进一步掌握。[结论]通过利用流式细胞仪对细胞凋亡的测定及结果分析,提高了仪器利用率,巩固了理论课内容,是本科细胞生物学实验的理想选题。

[Objective] To provide a new topic for cytobiology experiment in undergraduate education and improve the utilization of flow cytometry.[Method] According to the basic knowledge of cytobiology,the cell apoptosis was determined by different methods with the flow cytometry.[Result] The experimental results concluded by different methods can all reflect and interpret the phenomenon of cell apoptosis,which is helpful to students＇ further insight into cell properties and flow cytometry.[Conclusion] The use of flow cytometry for the determination and analysis of cell apoptosis can improve the equipment utilization and consolidate the theoretical course content,which turns out to be an ideal topic for undergraduate cell biology experiment.