解毒护肝饮药物血清对异烟肼致HepG2坏死和凋亡的干预

Effect of Jiedu Hugan Decoction containing serum on the death and apoptosis of HepG2 which were damaged by isoniazid

目的 观察解毒护肝饮药物血清对异烟肼致人肝癌细胞株(human hepatocellular carcinoma cell lines,HepG2)病变的干预,并分析其机制。方法 制备解毒护肝饮和甘草酸二铵的药物血清,建立异烟肼致HepG2细胞损伤模型,以解毒护肝饮和甘草酸二铵药物血清加入HepG2培养液,观察对HepG2坏死和凋亡的干预,以及对HepG2 Fas/FasL表达的影响,以评价解毒护肝饮的疗效机制。结果 解毒护肝饮药物血清对异烟肼致HepG2损伤有明显抑制作用,可以减少HepG2细胞凋亡和坏死,并减少其Fas/FasL表达,效果优于甘草酸二铵胶囊组。结论 以清热利湿解毒兼养阴化瘀为治法的解毒护肝饮有确切的减少异烟肼致HepG2坏死和凋亡的作用,其抗细胞凋亡作用与减少HepG2的Fas/FasL的表达有关。

ObjectiveTo observe the effect of Jiedu Hugan Decoction containing serum on HepG2 damaged by isoniazid,and reveal the mechanism of it.MethodsEstablish iv votro HepG2 cell damaged by isoniazid culture experiment model.The serum was collected from the rats using Jiedu Hugan Decoction and Gancaosuaneran and incubated with the HepG2 model.After 24 hours,observe the death and apoptosis of HepG2.And the expressions of Fas/FasL were measured by flow cytometry.ResultsCulture experiment model of iv votro HepG2 cell damaged by isoniazid was established successfully.Jiedu Hugan Decoction containing serum significantly inhibited apoptosis and necrosis of the HepG2 cells and the expression of Fas/FasL.The curative effect of this is better than control group which were given Gancaosuaneran decoction containing serum.ConclusionJiedu Hugan Decoction according to qingre lishi jiedu can evidently reduce the hepatitis by decrease the expression of the factor associated with apoptosis.So it can improve the syndromes and experimental index of hepatitis induced by anti-tubercular drug.