知母治疗湿热痹证有效物质组纯化工艺优选

Optimization of Purification Technology for Effective Material Group from Anemarrhena asphodeloides for Damp Arthralgia

目的:优选知母治疗湿热痹症有效物质组纯化工艺参数。方法:以芒果苷为指标,HPLC测定含量,对8种不同类型树脂进行考察,优选最佳树脂型号及其纯化条件。结果:确定最佳纯化条件为HPD-400型大孔树脂,加2 BV提取液进行吸附,分别用3 BV水,6 BV 30%乙醇洗脱。纯化后芒果苷纯度比原药液中含量提高6.6倍,总固体物减少87.3%。结论:纯化后药液中多糖等杂质明显减少,以芒果苷为代表的皂苷类成分得以较好的保留,为知母治疗湿热痹证研究提供前期研究基础。

Objective：To optimize purification technology parameters of effective material group from Anemarrhena asphodeloides for damp arthralgia in clinical.Method： With mangiferin as index,eight different types macroporous resins were investigated,optimum resin and its purification conditions were optimized.Result： Optimum macroporous resin was HPD-400 resin,and its purification conditions were as follows：added 2 BV extract liquid to adsorption,then eluted by 3 BV water,and eluted with 6 BV 30% ethanol.After purified,purity of mangiferin increased 6.6 times as original liquid,total solids reduced 87.3%.Conclusion： After purified,polysaccharide and other impurities reduced significantly in medicine liquid,mangiferin（as represent of saponins） was preserved better,this study provided preliminary research base for treatment of damp arthralgia with A.asphodeloides.