美洲大蠊醇提取物的木瓜蛋白酶酶解工艺

Enzymatic Process of Ethanol Extract from Periplaneta americana by Papain

目的:优选木瓜蛋白酶对美洲大蠊醇提取物的酶解工艺。方法:以水解度为指标,通过单因素和正交试验,分别考察酶解温度、酶解时间、pH、加酶量、底物浓度对木瓜蛋白酶酶解美洲大蠊醇提取物的影响。结果:木瓜蛋白酶酶解美洲大蠊醇提取物的最佳工艺条件为酶解温度55℃,时间3 h,pH 7.0,加酶量8 400 U·mg-1,底物浓度0.5%。在此条件下,水解度可达9.87%。酶解后的样品相对分子质量变小,相对分子质量分布变宽。结论:优选方法稳定,操作简便,为进一步研究美洲大蠊提供依据和基础。

Objective：To study on enzymatic technology of ethanol extract from Periplaneta americana by papain and screen out optimum enzymatic technology.Method： Taking degree of hydrolysis as index,effect of enzymatic temperature,enzymatic time,pH,the amount of papain and substrate concentration on papain were investigated by single factor experiment and orthogonal test.Result： Optimum enzymatic technology conditions of papain was determined as follows：enzymatic temperature 55 ℃,enzymatic time 3 h,pH 7.0,the amount of papain 8 400 U·mg-1,substrate concentration 0.5%.Under these conditions,degree of hydrolysis could reach to 9.87%.Molecular weight of sample were smaller and molecular weight distribution were wider after enzymolysis.Conclusion： This optimized method was stable,easy to operate,and provided basis and foundation to further study of P.americana.