摘要
目的:对荚果蕨贯众提取工艺进行优化。方法:采用L9(34)正交设计,以总黄酮得率为考察指标,紫外比色法测定含量,考察乙醇体积分数、料液比、提取时间和提取次数4个因素对提取率的影响。结果:最佳提取工艺为12倍量70%乙醇提取1.0 h,2次。结论:优选的荚果蕨贯众提取工艺稳定、可靠。
Objective:To optimize extraction process of Matteuccia struthiopteris.Method: Orthogonal test was carried out to investigate influences of the concentration of ethanol,material-liquid ratio,extraction time and extraction times on extraction with yield of total flavonoids as index.Result: Optimum extraction technology was as follows: the concentration of ethanol was 70%,ratio of material-solvent was 1∶ 12(g·mL-1),extracted 2 times with 1 h per time.Conclusion: Optimized extraction process was stable and reasonable.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第8期25-27,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中国中医科学院自主选题项目(Z02063)
关键词
荚果蕨贯众
正交设计
提取工艺
Matteuccia struthiopteris
orthogonal design
extraction technology