正交试验法优选舒郁煎膏水提工艺

Optimization of Water Extraction Process of Shuyu Decoction by Orthogonal Test

目的:优选舒郁煎膏的水提工艺。方法:以浸膏得率和栀子苷含量为评价指标。栀子苷含量采用高效液相色谱法测定,采用L9(3)4正交试验设计筛选舒郁煎膏的最佳水提工艺条件。结果:最佳水提工艺为加12倍量水,浸泡1 h,煎煮3次,分别煎煮1.5,1,1 h。结论:优选得到的水提工艺稳定可行,适合于工业化生产,为舒郁煎膏的制剂研究提供参考。

Objective：To optimize water extraction process of Shuyu decoction.Method： With extract yield of aqueous extract and the content of geniposide as indexes,the content of geniposide was determined by HPLC,optimum water extraction conditions was selected by L9（3）4orthogonal design.Result： Optimal water extraction process of Shuyu decoction was：12 times the amount of water,soaked 1 h,extracted 3 times with 1.5,1,1 h,respectively.Conclusion： Optimized water extraction process was stable,feasible and suited for industrial production.It could settle foundation for preparation study of Shuyu decoction.