白土苓中大泽明素与总黄酮的含量测定

Studies on Content Determination of Macrozamin and Total Flavonoids in Rhizoma Heterosmilacis

目的:考察白土苓药材中大泽明素、总黄酮的含量。方法:分别采用高效液相法和紫外-可见分光光度法对白土苓药材中的大泽明素和总黄酮进行了含量测定。结果:白土苓药材中大泽明素的平均含量为0.48%,在0.148 8～0.744 1μg呈良好的线性关系(r=0.999 4),回收率为101.07%,RSD 1.27%;总黄酮的平均含量为0.52%,橙皮苷在3.708～25.956 mg·L-1与吸光度呈良好的线性关系(r=0.999 9),回收率为99.44%,RSD 1.46%。结论:该方法准确、简便,为科学评价白土苓药材的质量提供依据。

Objective：To determine the content of macrozamin and total flavonoids in Rhizoma Heterosmilacis.Method： The content of macrozamin and total flavonoids was determined separately by HPLC and ultraviolet spectrophotometry（UV）.Result： The average content of macrozamin in Rhizoma Heterosmilacis was 0.48%.The calibration curve of macrozamin showed good linearity in the range of 0.148 8-0.744 1 μg（r=0.999 4）,with recovery rate 101.07% and RSD 1.27%.The average content of total flavonoids was 0.52%.The calibration curve of hesperidin showed good linearity in the range of 3.708-25.956 mg·L-1（r=0.999 9）,with recovery rate 99.44% and RSD 1.46%.Conclusion： The methods were approved accurate and simple.They could be used to scientifically evaluate Rhizoma Heterosmilacis.