清浊安中汤对慢性胃炎脾胃湿热证大鼠模型环氧合酶-2的影响

The Influence of Qingzhuo Anzhong Decoction on COX-2 in Rat Model with Splenogastric Hygropyrexia Syndrome of Chronic Gastritis

目的:探讨清浊安中汤治疗脾胃湿热证作用机制及脾胃湿热证大鼠模型的建立。方法:50只大鼠随机分为正常对照组、胃炎脾虚证组、胃炎湿热证组、清浊安中汤高、低剂量防治组,不同组用不同药物灌胃,连续20 d。采用"病证结合"方法,以2%水杨酸钠+高脂高糖+人工气候箱复制脾胃湿热证大鼠模型,并用清浊安中汤对脾胃湿热证大鼠模型防治,常规HE胃黏膜病理组织染色、免疫组化(SABC)法检测环氧合酶-2(COX-2)蛋白表达。结果:脾胃湿热证组10只大鼠均有轻-重度炎症,COX-2蛋白表达为0.289±0.036;脾胃湿热证大鼠模型表现出的症状、体征与临床相符,胃黏膜炎症较正常组重而与脾虚证组无差异,COX-2蛋白表达大于脾虚组与正常组(P<0.05),COX-2蛋白表达与炎症程度呈正相关,相关系数为0.662,P<0.01,清浊安中汤防治组对此有改善治疗作用。结论:大鼠模型符合脾胃湿热证属性,调节COX-2蛋白表达可能是清浊安中汤治疗脾胃湿热证作用机制之一。

Objective：To investigate studying the effect of Qingzhuo Anzhong decoction on cyclooxygenase-2（COX-2） in rat model with splenogastric hygropyrexia syndrome（SHS） of chronic gastritis.Method： The SHS and damp-heat syndrome modes were manufactured by 2%sodium salicylate and artificial climate box and Qingzhuo Anzhong decotion.The inflammation situation of gastric mucosa were observed by using gastroscope and common pathological staining method.The protein expression of gastric mucosa COX-2 was detected by immunohisochemistry.Result： The gastritis damp-heat syndrome rats model appeared symptom,physical sign with clinic.The rat gastritis splenic asthenia group and gastritis moist heat group are quite serious in gastric mucosa inflammation,by comparing with the normal group,the difference is significant（P0.05）.SHS group＇ COX-2 protein expresses strongly is splenic asthenia group and the normal group（P0.05）.The inflammation degree and COX-2 protein expression appears positive correlation（P0.01）.Qingzhuo Anzhong decotion can improve above-mentioned condition after applying it.Conclusion： Rat model consistents with attribute to SHS.Adjusting COX-2 protein expresses may be one of mechanism of action on treating SHS with Qingzhuo Anzhong decotion.