五淋散胶囊药效学研究

Phamacodynamic Studies on Wulinsan Capsule

目的:五淋散胶囊的抗炎作用实验。方法:体外观察其对相关细菌的抗菌作用及溶石作用,体内将小鼠随机分为正常、阳性药和五淋散胶囊高、中、低剂量组(14.19,7.10,3.55 g·kg-1),大鼠随机分为正常、模型、阳性药,五淋散胶囊高、中、低剂量组(9.93,4.97,2.48 g·kg-1),ig给药。采用巴豆油致小鼠耳廓肿胀、小鼠腹腔毛细血管通透性、大鼠棉球肉芽肿观察其抗炎作用;大鼠代谢笼法观察其利尿作用;乙二醇和氯化铵诱导大鼠肾结石观察其抗肾结石作用;环磷酰胺致大鼠出血性膀胱炎及角叉菜胶致大鼠非细菌性前列腺炎观察其对泌尿系统炎症的影响。结果:五淋散胶囊对金黄色葡萄球菌、表皮葡萄球菌、大肠埃希菌、变形杆菌和白色念珠菌的MIC分别为18.5,74,295,18.5,295 g.L-1;14.19,7.10 g·kg-1剂量组对小鼠耳肿胀和毛细血管通透性增加有明显抑制作用(P<0.05或P<0.01);9.93,4.97,2.48 g·kg-1剂量组均能减轻大鼠棉球肉芽肿质量(P<0.01);4.97 g·kg-1剂量组能增加水负荷大鼠6 h时间段的尿量(P<0.05);高剂量组(20%)的五淋散溶液能使结石质量减轻(P<0.05);9.93 g·kg-1剂量组能减少大鼠草酸钙结石的结石形成率,改善肾脏病变;9.93,4.97,2.48 g·kg-1剂量组均能明显减轻大鼠膀胱的出血和水肿程度(P<0.05);4.97 g·kg-1剂量组能有效改善角叉菜胶所致的大鼠急性前列腺炎病变(P<0.05)。结论:五淋散胶囊具有抗菌、抗炎、利尿、促进肾功能恢复和降低结石形成的作用。

Objective：To study the main pharmacological effects of Wulinsan capsule.Method：The effects of Wulinsan capsule on dissolved stones and antibacterial activity in vitro were observerd;mice were randomly divided into five groups： normal group,positive group,high dose group,middle dose group and low dose group of Wulinsan capsule（14.19,7.10,3.55 g·kg-1）.Rats were randomly divided into six groups： normal group,model group,positive group,high dose group,middle dose group and low dose group of Wulinsan capsule（9.93,4.97,2.48 g·kg-1）,ig.The anti-inflammatory effects were studied by following midels： the corton oil induced swelling in mice,the capillary permeability in abdominal cavity in mice and cotton ball granuloma reaction in rats.The diuretic effects were studied by metabolism cage of rats;the renal calculus model induced by ethlene glycd and annonia chloride in rat was applied to observe the anti-renal calculus effct,the hemorrhagic cystitis coused by cyclophosphamide in rats and the non-bacterial prostatitis coused by carrageenan in rats were applied to observe the anti-inflammation of the urinary.Result：Wulinsan capsule could inhibit the growth of Staphyococcus aureus,Staphyococcus epidermidis,Escherichia coli,Proteus,Candida albicans,MIC was 18.5,74,295,18.5,295 g·L-1,Wulinsan capsule at doses of 14.19,7.10 g·kg-1 could also obviously inhabit the increase of capillary permeability and swelling ear in mice（P0.05 or P0.01）,and could reduce the weigh of the cotton ball granuloma in rats（P0.01）at all doses,increased the urine output of water load for 6 h period in rat（P0.05）at the dose of 4.97 g·kg-1,20% solution of Wulinsan could reduce the weigh of stone（P0.05）,lowered the rate of rat calcium oxalate stone formation and improve kidney change at the dose of 9.93 g·kg-1,significantly reduced the bleeding and the degree of edema in bladderand at all doses（P0.05）,improve the acute prostatitis lesions caused by carrageenan in rats at the dose of 4.97 g·kg-1（P0.05）.Conclusion： Wulinsan capsule has the effects of antibacterial,anti-inflammatory,diuresis,promoting the recovery of kidney and reducing the stone formation.