普胃丸对胃溃疡复发大鼠胃组织VEGF和bFGF表达的影响

The Effect of Puwei Pill on Expression of VEGF and bFGF in Gastric Tissue in Gastric Ulcer Recurrence Rats

目的:观察普胃丸(PWP)对胃溃疡(GU)复发过程中大鼠胃组织中血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)表达的影响。方法:将SD大鼠随机分为6组,即正常对照组、假手术组、模型未复发组、模型复发组、奥美拉唑组、PWP组。采用醋酸烧灼法建立大鼠慢性GU模型,造模24 h后各组大鼠ig给药,奥美拉唑组大鼠3.6 mg·kg-1,1次/d,PWP组大鼠40 mg·kg-1,1次/周,其余各组每天给予等量蒸馏水。于造模第90天,除模型未复发组外,其余各组大鼠均ip白细胞介素-1β(IL-1β)1μg.kg-1,诱导大鼠GU复发。在溃疡复发后48 h测定GU复发率,并分别采用免疫组化法和Westernblot法检测胃组织中VEGF和bFGF的表达。结果:正常对照组、假手术组和模型未复发组均未见溃疡复发,模型复发组溃疡复发率为83.3%,PWP组和奥美拉唑组溃疡复发率分别为11.1%和33.3%,明显低于模型复发组(P<0.01);模型复发组VEGF和bFGF的平均阳性密度分别为127.03±13.47,122.40±23.17;平均吸光度分别为126.78±13.62,121.16±20.09,其值较正常对照组、假手术组和模型未复发组高(P<0.01),但明显低于PWP组和奥美拉唑组(P<0.01)。结论:PWP可通过增加胃组织中VEGF和bFGF的表达来降低溃疡复发率。

Objective：To investigate the effects of Puwei Pill（PWP） on expressions of vascular endothelial growth factor（VEGF） and basic fibroblast growth factor（bFGF） in gastric tissue in gastric ulcer recurrence rats.Method： The SD rats were randomly divided six groups： the normal control,the sham,the model without recurrence,the model with recurrence,the omeprazole and PWP groups.The chronic gastric ulcer model was built by using acetic acid.The rats in each group were administrated by ig at 24 h after modeling.The rats in the omeprazole group were ig given 3.6 mg·kg-1,once a day,the rats in the PWP group were ig given 40 mg·kg-1,once a week,and the rats in other groups were ig distilled water.To induce the gastric ulcer recurrence,the rats in other group except for the model without recurrence were ip given interleukin-1β（IL-1β） at 90 d after modeling.The recurrence rate was calculated at 48 h after inducing ulcer recurrence.The VEGF and bFGF in gastric tissue were determined by immunohistochemical staining and western blot methods.Result： The ulcers were not found in the normal control,the sham and the model without recurrence groups.The recurrence rate was 83.3% in the model recurrence group,but it was lower in PWP and omeprazole groups（P0.01）,which was separately 11.1% and 33.3%.Compared with the model with recurrence group,the average positive dense of VEGF and bFGF was separately 127.03±13.47 and 122.40±23.17,and the average value of optical-density was separately 126.78±13.62,121.16±20.09,which was higher than in the normal control,the sham and the model without recurrence groups（P0.01）,but was lower than in PWP and omeprazole groups（P0.01）.Conclusion： PWP could decrease recurrence rate of gastric ulcer by increasing expression of VEGF and bFGF in gastric tissue.