蒲公英水提物对大鼠溃疡性结肠炎的实验研究

Effect of Dandelion Extract in Ulcerative Colitis Rats

目的:研究蒲公英水提物对大鼠溃疡性结肠炎疗效及机制。方法:将60只大鼠随机分成正常对照组(等容蒸馏水),模型组(等容蒸馏水),柳氮磺胺嘧啶(SASP,400 mg·kg-1)组,蒲公英水提物高、中、低剂量组(150,100,50 mg·kg-1)。2,4,6-三硝基苯磺酸(TNBS)/乙醇溶液100 mg·kg-1灌肠制备溃疡性结肠炎大鼠模型,造模10 d后,ig给药,2 mL/次,1次/d,连续14 d,实验第14天断头取血和结肠标本,观察黏膜大体形态及组织学改变,酶标记免疫吸附测定法(ELISA)法测定血清白细胞介素-6(IL-6),白细胞介素-10(IL-10),肿瘤坏死因子-α(TNF-α)水平,SP免疫组织化学染色法检测结肠组织转录因子核因子-κB(NF-κB)p65表达。结果:蒲公英水提物高、中、低剂量组与模型组比较,大鼠组织大体形态评分显著下降,分别为(5.11±0.43),(6.11±0.31),(7.01±0.38),(7.89±0.31)分(P<0.05)。病理组织学评分显著下降,分别为(8.11±1.23),(9.37±1.12),(10.12±1.32),(12.17±1.56)分(P<0.05)。血清IL-6显著降低,分别为(68.53±18.21),(71.41±13.38),(80.24±15.29),(89.91±19.13)ng·L-1(P<0.05)。IL-10水平显著升高,分别为(35.61±4.19),(29.39±4.91),(21.83±5.63),(19.51±4.98)ng·L-1(P<0.05)。TNF-α显著下降,分别为(36.27±8.35),(46.29±9.32),(64.18±8.98),(71.27±8.81)ng·L-1(P<0.05,P<0.01)。NF-κB p65表达降低,分别为(0.301±0.038),(0.351±0.053),(0.389±0.041),(0.411±0.086)%,(P<0.05,P<0.01)。高剂量组在各项检测指标与SASP组之间差异无显著性。结论:蒲公英水提物治疗溃疡性结肠炎具有一定的疗效。

Objective：To observe therapeutic effect and mechanism of Dandelion extract in rat ulcerative colitis（UC） model.Method： Sixty rats were randomly divided into control group,model group,sulfasalazine group（SASP,400 mg·kg-1）,high-dose,medium-dose and low-dose group of Dandelion extract（50,100,150 mg·kg-1）.Trinitrobenzene sulphonic acid（TNBS,100 mg·kg-1） was instilled into the colon of rats to induce ulcerative colitis.These groups were given relevant drugs with 2 mL,once a day for 14 d.On the day 14,the rats were sacrificed to collect blood and colon specimens.The morphology and histological changes of mucous membrane were observed,ELISA was performed to detect the levels of interleukin-6（IL-6）,interleukin-10（IL-10） and tumor necrosis factor-α（TNF-α）,and streptavidin-perosidase（SP） was performed to detect expression of nuclear factor-κB（NF-κB） p65.Result： Compared with model group（7.89±0.31,12.17±1.56）,the Dandelion extract at high-dose,medium-dose and low-dose could reduce the morphologic score of mucous membrane significantly（5.11±0.43）,（6.11±0.31）,（7.01±0.38）,（P0.05）,and histological changes was（8.11±1.23）,（9.37±1.12）,（10.12±1.32）respectively,（P0.05）.The level of IL-6 and TNF-α was decreased（68.53±18.21）,（71.41±13.38）,（80.24±15.29）,（36.27±8.35）,（46.29±9.32）,（64.18±8.98） ng·L-1 compared with model group（89.91±19.13）,（71.27±8.81） ng·L-1（P0.05,P0.01）.Expression of NF-κB p65 was reduced from（0.411±0.086）% to（0.301±0.038）%,（0.351±0.053）%,（0.389±0.041）%（P0.05,P0.01）.But there was no obvious difference between Dandelion extract high-dose group and SASP group.Conclusion： These results indicated that Dandelion extract has effects on ulcerative colitis.