补肾填精方对再生障碍性贫血大鼠模型IL-11及EPO影响的研究

Experimental Study on IL-11 and EPO in Aplastic Anemia Rat Treated with Tonifying Kidney and Replenishing Essence Prescription.

目的:观察补肾填精方对再生障碍性贫血(aplastic anemia,AA)模型大鼠调控因子白细胞介素-11(interleukin-11,IL-11)及红细胞生成素(erythropoietin,EPO)的影响。方法:Wistar大鼠随机分为空白对照组、模型生理盐水组、补肾填精中药组、再障生血片组共4组各12只,对照组正常饲喂不做干预;其他3组以5-氟尿嘧啶注射液(5-FU)与马利兰合用诱发建立模型后,补肾填精中药组及再障生血片组分别给予补肾填精中药10 g·kg-1及再障生血片混悬液0.015 g·kg-1,连续4周,模型组予等量生理盐水灌胃。结果:①治疗组的白细胞(WBC)、血红蛋白(Hb)、红细胞(RBC)及血小板(PLT)较模型组均有不同程度增高,差异有统计学意义(P<0.05)。其中补肾填精中药组的WBC和PLT明显升高,差异有统计学意义(P<0.01)。②治疗组IL-11含量较模型组升高,差异有统计学意义(P<0.05)。③治疗组EPO含量较模型组降低,差异有统计学意义(P<0.05)。结论:补肾填精方能改善模型大鼠的骨髓造血功能,可改善细胞因子IL-11及EPO介导的分泌异常,促进骨髓造血功能的恢复。

Objective：To observed the effect of tonifying kidney and replenishing essence prescription（TKREP） on two regulation factors interleukin-11（IL-11） and eryhropictin（EPO） in Aplastic Anemia（AA） rats.Method： Forty eight clean grade Wistar rats were randomly divided into 4 groups,namely normal control group,AA model group,TKREP group and the aplastic blood-forming tablet（ABFT）group.The normal control group was fed with normal foods and vihicle.The other three groups were given the fluorouracil（5-FU） plus Myleran to induce AA model of rat.After that,the TKREP group and ABFT group rats were fed with TKREP（10 g·kg-1·d-1）and ABFT（0.015 g·kg-1·d-1） for 4 weeks.While,the AA model group was fed 0.9% NaCl.Result：①Compared to model group,the numbers of red blood cells and hemoglobin in treatment group were decreased,the difference was statistically significant（P0.05）;the numbers of white blood cells and platelet in TKREP group were significantly decreased,the difference was statistically significant（P0.01）;②EPO levels were elevated in treatment group,the difference was statistically significant（P0.05）;③while the IL-11 level was increased slightly,the difference was statistically significant（P0.05）.Conclusion： From point view of tonifying kidney,the TKREP could significantly improve bone marrow hematopoietic function against 5-FU plus Myleran,to a certain extent,effectively improve abnormal secreteing of regulation factors（IL-11 and EPO） and encouraged the bone marrow hematopoietic function＇s recover.