RP-HPLC法测定石榴汁中鞣花酸的含量

Determination of ellagic acid in the pomegranate juice by RP-HPLC

目的:建立检测石榴汁中鞣花酸的含量测定方法。方法;采用高效液相色谱法,ArcusEP-C18(250mm×4.6mm,5μm)色谱柱,以甲醇-0.1%TFA为流动相梯度洗脱,流速1.0mL/min,检测波长254nm。结果:鞣花酸0.004～0.064μg之间线性关系良好,平均加样回收率为99.32%。结论:方法精密可靠,可作为检测石榴汁中鞣花酸含量的方法。

Objective：To establish HPLC method for determination of ellagic acid in the pomegranate juice.Method：The RP-HPLC was carried out on Arcus EP-C 18（250 mm×4.6 mm,5 μm）.The gradient elution was performed by a mobile phase consisted a mixture of methanol and 0.1% TFA at a flow rate of 1.0 mL/min,and detection wavelength was at 254 nm.Results：The linear range was 0.004～0.064 μg for ellagic acid.The average recoveries was 99.32%.Conclusion：The method is accurate and reproducible for quality determination of ellagic acid in pomegranate juice.