长足大竹象成虫体表信息化学物质的提取和鉴定

Extraction and identification of cuticular semiochemical components of Cyrtotrachelus buqueti Guerin-Meneville(Coleoptera:Curculionidae)

为了确定我国南方竹林主要害虫长足大竹象Cyrtotrachelus buqueti Guerin-Meneville雌虫是否存在诱引雄虫的化学信息素以及所释放的相关化学信息素的成分,本研究利用风洞和"Y"型嗅觉仪测试到长足大竹象雄虫对雌虫及雌虫体表提取化合物具有正趋向行为反应,随后用GC-EAD和GC-MS对雌虫体表提取化合物进行了分析。在GC-EAD分析时发现雄虫触角对雌虫体表粗提液和体表物质标准品化合物的触角电位反应时有5个GC峰有电生理活性;通过质谱法确定这5个电生理活性GC峰的化学成分分别为苯酚、己酸乙酯、2-壬酮、壬醛和十五酸甲酯。风洞和GC-EAD分析表明,这5种成分的标准品化合物对长足大竹象雄虫具有诱引生物活性和触角电生理活性。结果说明长足大竹象雌虫体表粗提液中存在的苯酚、己酸乙酯、2-壬酮、壬醛和十五酸甲酯是长足大竹象成虫的化学信息素物质。

The aim of this study is to identify the semiochemicals of the female weevil,Cyrtotrachelus buqueti Guerin-Meneville,an important bamboo forest weevil pest in Southern China.The behavioral responses of male weevils to the crude cuticular extracts from virgin female weevils in a flight tunnel was analyzed,and the results showed that male weevils had positive response to the cuticular extracts as well as to virgin female weevils.The sensitive reaction of male weevil＇s antenna to the crude cuticular extracts from virgin female weevils was detected by GC-EAD,and the components of the cuticular extracts that consistently elicited antennal responses were identified by GC-MS.The results indicated that male weevil＇s antenna had sensitive reaction to synthetic standard chemicals of five components in the female extracts,i.e.,phenol,ethyl hexanoate,2-nonanone,nonanal,and pentadecanoic acid methyl ester.Flight tunnel and GC-EAD studies showed that the five synthetic chemicals were also bioactive to male weevils,suggesting that phenol,ethyl hexanoate,2-nonanone,nonanal,and pentadecanoic acid methyl ester are semiochemicals of C.buqueti.