车前子综合化学模式识别研究

Comprehensive chemical pattern recognition of Pantagirus Semen

目的:运用高效液相色谱指纹图谱相似度评价法、系统聚类分析法和主成分分析法建立车前子药材的综合化学模式识别方法。方法:采用反相高效液相法,紫外检测器,Diamonsil C18柱(4.6 mm×250 mm,5μm),乙腈-0.5%乙酸水溶液为流动相梯度洗脱,建立车前子的HPLC-UV指纹图谱,并以24批药材共有色谱峰峰面积为依据,建立其系统聚类分析方法及主成分分析方法。结果:指纹图谱法可以区别不同品种及产地车前子,系统聚类分析法和主成分分析法分析结果基本一致,但稍有差异。结论:所建立的综合化学模式识别方法可用于车前子的质量控制及品种评价。

Objective： To establish a method of comprehensive chemical pattern recognition of plantain seed via HPLC fingerprint,principal component analysis（PCA） and cluster analysis.Method： The chromatographic separation was performed on a C18 column（4.6 mm×250 mm,5 μm）.The mobile phase was a mixture of acetonitrile-0.5% acetic acid aqueous solution in gradient elution.The HPLC fingerprint of ethyl acetate fraction of 24 batches Plantaginis Semen from different habits and varieties was set up and 10 common peaks were obtained.Result： The result of the principal component analysis（PCA） and cluster analysis is similar but there is disparity between them.Conclusion： The method could be used for the quality control and comprehensive evaluation of Plantaginis Semen.