HPLC法测定穿山龙中伪原薯蓣皂苷、薯蓣皂苷和延龄草苷的含量

HPLC simultaneous determination of pseudoprotodioscin,dioscin and trillin in Dioscoreae Nipponicae Rhizoma

目的:建立反相高效液相色谱法同时测定穿山龙中伪原薯蓣皂苷、薯蓣皂苷和延龄草苷的含量。方法:采用Zorbax SB-C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈(A)-水(B),梯度洗脱(0～18 min,25%A线性变为36%A;18～40min,36%A线性变为90%A;40～55 min,90%A保持不变),流速1.0 mL.min-1,检测波长210 nm,柱温30℃。结果:伪原薯蓣皂苷、薯蓣皂苷和延龄草苷的色谱峰面积与浓度呈良好的线性关系,线性范围分别为9.80～588.0μg.mL-1(r=0.9994),20.20～1212μg.mL-1(r=0.9995),5.330～319.8μg.mL-1(r=0.9999);平均回收率(n=9)分别为97.3%,99.4%,97.6%。结论:该方法简便、准确,为穿山龙药材的质量评价提供了方法。

Objective：To establish a quantitative method for the determination of pseudoprotodioscin,dioscin and trillin in Dioscoreae Nipponicae Rhizoma.Methods：The determination was performanced on Zorbax SB-C18（250 mm×4.6 mm,5 μm） column with the mobile phase consisting of acetonitrile（A） and water（B） with gradient elution mode at a flow rate of 1.0 mL·min-1.The following gradient procedure was used：0-18 min,25%A→36%A;18-40 min,36%A→90%A;40-55 min,90%A;Detection wavelength was 210 nm,the column temperature was 30 ℃.Results：The method had good linear relationship within the ranges of 9.80-588.0 μg·mL-1 for pseudoprotodioscin（r=0.9994）;20.20-1212 μg·mL-1 for dioscin（r=0.9995）;5.330-319.8 μg·mL-1 for trillin（r=0.9999）.The average recoveries（n=9） were 97.3% for pseudoprotodioscin,99.4% for dioscin,and 97.6% for trillin.Conclusion：The method is simple and accurate.It provides a reliable way for evaluating the quality of Dioscoreae Nipponicae Rhizoma.