交联温度对京尼平交联壳聚糖/藻酸盐组织工程支架的影响

Effect of temperature on cross-linking of chitosan/alginate scaffolds with genipin

[目的]通过对壳聚糖/藻酸盐复合支架材料表面结构、降解率、细胞毒性、孔隙率、含水量以及生物力学性能变化的研究,探究交联温度对京尼平交联壳聚糖/藻酸盐组织工程支架的影响。[方法]运用冷冻干燥法制备壳聚糖/藻酸盐支架材料,分别在4℃、25℃、37℃条件,于0.5%京尼平溶液中交联24 h,作为实验组。以未用京尼平交联的支架材料作为对照组,评价支架材料降解率、细胞毒性、孔隙率、含水量以及生物力学性能的特点。[结果](1)伴随交联温度升高,支架材料的颜色从浅紫色变为紫黑色,未交联的支架材料为白色;(2)降解率4周后在4℃为21.54%±3.07%、25℃组为9.9%±1.2%、37℃组为8.98%±0.79%。其中,37℃和25℃组抗降解能力优于4℃组(P<0.05)。各实验组均优于对照组(P<0.01);(3)弹性模量在4℃组为0.84±0.55,25℃组为1.44±0.06,37℃组为1.53±0.02,对照组为0.79±0.16。对照组与25℃组及37℃组之间差异有统计学意义(P<0.05);(4)孔隙率、含水量、细胞毒性各实验组间未见明显差异。[结论]随着交联温度的提高,支架材料的抗降解能力、抗拉伸性能增加,且对支架材料的结构、细胞毒性、孔隙率以及含水量无明显影响。

[ Objective] Through research of the change of the chitosan/alginate scaffolds material surface structure, degra- dation rate, cytotoxicity, porosity, water content and biomechanics properties, to explore cross-linking temperature of genipin crosslinked effects of chitosan/alginate scaffolds for tissue engineering. [ Method] The chitosan / alginate scaffolds of tissue en- gineering was made by freeze-drying, respectively, in 4 ℃ ,25 ℃ ,37 ℃ conditions, at 0.5 % solution of genipin crosslinked 24 h, as the experimental group. The scaffolds of not cross-linked with genipin as a control group to evaluate the degradation rate of scaffolds, cytotoxicity, porosity, water content and biomechanics properties. [ Result ] （ 1 ） The color of scaffolds material changed from light purple to purplish black, according with the rise of the cross-linking temperature, however, not cross-linked scaifold material was white. （2）The degradation rate after 4 weeks at 4℃ group was 21.54% ± 3.07% ,25℃ group was 9.9% ±1. 2% ,37℃ group was 8.98% ±0.79%. Among them,the 37℃group of degradation resistant capacity was better than the 4℃group （P 〈0.05 ）. The experimental groups were better than the control group （P 〈 0.01 ）. （3）Elastic nmdulus at 4 ℃ group was 0.84 ±0.55,25℃ group was 1.44 ±0.06,37℃ group was 1.53 ±0.02. 37℃ group and 25℃ group of tensile properties better than control group （ P 〈 0.05 ）. （4） Porosity, water content, cytotoxicity between the experimental groups had no signifi- cant difference. [ Conclusion] With the cross-linking temperature increasing, scaffold degradation resistant capacity and tensile properties were strengthen, but on the scaffold material structure, cytotoxicity, porosity and water content were not altered signifi- cantly.