氮与pH互作对菊苣4项生理指标的影响

Interactive effects of nitrogen and pH on four physiological indicators of chicory

采用65.305,130.610和261.220mg.L-1三个质量浓度氮的改良Hoagland营养液及5.5、7.0和8.5三个pH水平完全随机等重复沙培试验,探讨了氮与pH互作对菊苣(Cichorium intybus)叶片叶绿素和丙二醛含量(MDA)及过氧化物酶(POD)和超氧化物歧化酶(SOD)活性等生理指标的影响。结果表明,1)在不同氮浓度处理时,菊苣叶片叶绿素和MDA含量无显著变化,而POD和SOD活性显著上升;2)在不同pH处理时,菊苣叶片叶绿素含量有显著变化,MDA含量无显著变化,而POD和SOD活性显著上升;3)氮与pH互作对菊苣生长有显著影响;菊苣叶片叶绿素含量在高氮低pH互作下最高,且随施氮量的增加持续增加;MDA含量在高氮低pH互作下最低,且在氮或pH胁迫时变化甚微;POD和SOD活性在高氮高pH互作下活性最低,且在受到氮或pH胁迫时变化显著。

A experiment was carried out to determine the interactive effects of nitrogen and pH with on chlorophyll and malondialdehyde（MDA） content,peroxidas（POD） and superoxide dismutase（SOD） activity of chicory（Cichorium intybus） by completely random combination of three nitrogen concentrations（65.305 mg·L-1,130.610 mg·L-1 and 261.122 mg·L-1） and three pH levels（5.5,7.0 and 8.5） in the sand culture.The results of this study showed the application of nitrogen did not affect the chlorophyll and MDA content but significantly affected the POD and SOD activity.The pH value significantly affected the chlorophyll but did not affected the MDA content,and increased the activity of POD and SOD.the interaction of nitrogen and pH had significant effect on chicory growth,indicating that the chlorophyll content were greatest with 65.305 mg·L-1 N and pH 5.5,and it increased as N concentration increased;the MDA content were lowest with 261.122 mg·L-1 N and pH 5.5,and it kept stable when only N or pH stress was applied;the POD and SOD activity were highest with 261.122 mg·L-1 N and pH 8.5,and it changed significantly when only N or pH stress was applied.