Activin B联合BMSCs不同移植方式对大鼠皮肤创伤愈合的治疗

The treatment of skin wound healing of rats by different transplantation approaches of BMSCs combination with Activin B

目的探讨BMSCs联合生长因子对治疗皮肤创伤最为有效的移植途径。方法分离并鉴定BMSCs,移植后活体成像仪及冰冻切片观察CFSE标记的BMSCs,统计创面愈合率,并进行HE染色观察伤口愈合。结果细胞表面标记物CD90表达呈阳性,CD80表达呈阴性。活体成像仪3、7d观察实验组大鼠皮肤创面均有荧光显示,而对照组无。术后各时间点BMSCs移植组创面愈合率均高于对照组(P<0.05),而不同移植方式相比差异无统计学意义。结论 Activin B联合BMSCs经表皮移植和尾静脉移植均可以迁移至皮肤创伤部位并参与皮肤创伤的修复,相对于尾静脉移植组,表皮移植组在创伤修复中更简便易行。

Objective To discuss the optimum transplantation approaches of BMSCs combination with Activin B on skin wound healing.Methods BMSCs were identified by immunocytochemistry.Residual situation of the CFSE-labeled BMSCs after transplantation was detected by the In Vivo imaging system and frozen section.Wounded areas were recorded and wound healing was observed by the paraffin section and HE staining.Results Positive expression of CD90 and negative expression of CD80 were observed by immunocytochemistry.Fluorescence of the wounded sites of rats in the experimental groups was observed by In vivo imaging system,but it didn＇t be observed in control group at the 7 and 14 days after skin wounding.Wound healing rate in the experimental group was increased in comparison with that in the control group（P0.05）,but wound healing rate in the different transplantation approaches had no significant difference.Conclusions BMSCs transplantation via the epidermal and the tail vein can both migrate to the wounded sites and promote the wound healing.Compared with the tail vein transplantation group,wound repair in the epidermal transplantation group was more simple and feasible.