透明质酸对人骨关节炎软骨细胞保护机制的探讨

Protective effects and mechanism of hyaluronic acid on human osteoarthritis chondrocytes

目的:探讨透明质酸对人骨关节炎软骨细胞的作用机制。方法:应用细胞培养、四唑盐比色实验检测细胞线粒体活性,流式细胞术检测线粒体膜电位、细胞凋亡百分率和细胞内游离钙离子浓度。结果:透明质酸能明显提高人骨关节炎软骨细胞线粒体活性,抬高线粒体膜电位,降低细胞凋亡率和胞内游离钙浓度。结论:透明质酸可抑制人骨关节炎软骨细胞线粒体膜电位的降低,从而具有稳定线粒体膜电位的作用,抑制细胞凋亡的发生,这种作用可能与其能抑制软骨细胞内钙超载有关。

Objective To study the effects and mechanism of hyaluronic acid on human osteoarthritis chondrocytes.Methods Mitochondrial activity was measured by methylthiazolyl tetrazolium test.Mitochondrial membrane protein（MMP）,apoptosis and i were measured by flow cytometry.Results Hyaluronic acid significantly increased MMP and mitochondrial activity,and decreased i and apoptosis rate in human osteoarthritis chondrocytes.Conclusions Hyaluronic acid might inhibit the declines of MMP and mitochondrial activity,and has an inhibitory effect on osteoarthritis chondrocytes apoptosis.The mechanism might be related to inhibiting intracellular calcium overload.