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慢病毒载体介导CD55基因抑制对BxPC-3细胞生长的影响

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摘要 目的:探讨CD55基因对胰腺癌BxPC-3细胞生长的影响。方法:用CD55-RNAi-LV转染BxPC-3细胞后以流式细胞术检测其对细胞凋亡、增殖、周期的影响。结果:CD55-RNAi-LV转染BxPC-3细胞后,与对照细胞相比,细胞凋亡率增高至(33.84±1.09)%(P<0.05);细胞增殖率降低至(30.06±0.97)%(P<0.05),细胞停滞于G1期为(65.41±3.59)%(P<0.05)。结论:胰腺肿瘤细胞系BxPC-3细胞中CD55基因表达可能对其生长有调控作用,有望成为胰腺癌治疗新靶点。
作者 何政 王敏 田锐 朱峰 李旭 林帆 秦仁义
机构地区 华中科技大学同济医学院附属同济医院胆胰外科
出处 《实用医学杂志》 CAS 北大核心 2012年第7期1048-1051,共4页 The Journal of Practical Medicine
基金 国家自然科学基金面上项目(编号:81071775) 国家自然科学基金青年科学基金项目(编号:81001068)
关键词 胰腺肿瘤 RNA干扰 CD55 慢病毒载体
分类号 R735.9 [医药卫生—肿瘤]
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参考文献10

  • 1Kleeff J,Michalski C,Friess H,et al.Pancreatic cancer:from bench to5-year survival[J].Pancreas,2006,33(2):111-118.
  • 2Schneider G,Siveke J T,Eckel F,et al.Pancreatic cancer:basicand clinical aspects[J].Gastroenterology,2005,128(6):1606-1625.
  • 3陈汝福,周泉波.胰头癌的各种手术方式评价[J].实用医学杂志,2010,26(10):1683-1684. 被引量：12
  • 4Juhl H,Helmiq F,Baltzer K,et al.Frequent expression ofcomplement resistance factors CD46,CD55,and CD59ongastrointestinal cancer cells limits the therapeutic potential ofmonoclonal antibody17-1A[J].J Surg Oncol,1997,64(3):222-230.
  • 5Gorter A,Meri S.Immune evasion of tumor cell usingmembrane-bound complement regulatory proteins[J].ImmunolToday,1999,20(12):576-582.
  • 6Atkinson J P,Oglesby T J,White D,et al.Separation of selffrom non-self in the complement system:a role for membranecofactor protein and decay accelerating factor[J].Clin ExpImmunol,1991,86(1):27-30.
  • 7Mustafa T,Klonisch T,Hombach-Klonisch S,et al.Expression of CD97and CD55in human medullary thyroidcarcinomas[J].Int J Oncol,2004,24(2):285-294.
  • 8Holla V R,Wang D,Brown J R,et al.Prostaglandin E2regulates the complement inhibitor CD55/decay-acceleratingfactor in colorectal cancer[J].J Biol Chem,2005,280(1):476-483.
  • 9Morgan J,Spendlove I,Durrant L G.The role of CD55inprotecting the tumour environment from complement attack[J].Tissue Antigens,2002,60(3):213-223.
  • 10夏金堂,徐波.胰腺癌非手术治疗的现状和展望[J].实用医学杂志,2010,26(10):1685-1687. 被引量：19

二级参考文献19

  • 1吴文铭,赵玉沛.胰腺癌物理治疗的进展及评价[J].消化外科,2005,4(1):73-76. 被引量：6
  • 2NCCN.Pancreatic adenocarcinoma V1 2009.available at http://www.nccn.org/professionals/physician_gls/f_guidelines.asp,03/26/2009.
  • 3Saif MW.Controversies in the adjuvant treatment of pancreatic adenocarcinoma[J].JOP,2007,8(5):545 -552.
  • 4Gebbia V,Maiello E,Giuliani F,et al.Second21ine chemotherapy in advanced pancreatic carcinoma:a multicenter survey of the Gruppo Oncologico Italia Meridionale on the activity and safety of the F0L2 F0X4 regimen in clinical practice[J].Ann Oncol,2007,18(Supp 1 6):124-127.
  • 5Louvet C,Labianea R,Hammel P,et al.Gemcitabine versus GEMOX(gemcitabine + oxaliplatin) in non resectable pancreatic adenocarcinoma:Interim results of the GERCOR/GISCADIntergroup Phase 1.Proc[J] Am Soc Clin Oncol,2003,22(abstr 1004):250.
  • 6Coquard R,Ayzac L,Gilly F,et al.Intraoperative radiotherapy in resected pancreatic cancer:feasibility and results[J].Radiother Oncol,1997,44(3):271-275.
  • 7Reni M,Panucci M,Ferreri A,et al.Effect of local control and survival of electron beam intraoperative irradiation for resectable pancreatic adenocarcinoma[J].Int J Radiat Oncol Biol Phys,2001,50(3):651-658.
  • 8Klinkenbijl J,Jeekel J,Sahmoud T,et al.Adjuvant radiotherapy and 5-fluorouracil after curative resection of cancer of the pancreas and periampullary region.Phase Ⅲ trial of the EORTC Gastrointestinal Tract Cancer Cooperative Group[J].Ann Surg,1999,230(8):776-784.
  • 9Delon-Martin C,Vogt C,Chignier E,et al.Venous thrombosis generation by means of high-intensity focused ultrasound[J].Ultrasound Med Biol,1995,21(1):113-119.
  • 10Homma H,Doi T,Mezawa S,et al.A novel arterial infusion chemotherapy for the treatment of patients with advanced pancreatic carcinoma after vascular supply distribution via superselectiveembolization[J].Cancer,2000,89(3):303-313.

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实用医学杂志
2012年 第7期

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