百合‘Siberia’בManissa’种间杂交后代的染色体核型分析

Karyotype Analysis of the Interspecific Hybrids between Oriental Hybrids Group 'Siberia' and OT Group 'Manissa'

以东方百合杂种系(Oriental hybrids group)品种‘Siberia’为母本,OT杂种系(interspecific hybrids betweenOriental and Longiflorum/OT group)品种‘Manissa’为父本杂交获得种间杂交F1代,对亲本及F1代株系的染色体数目和形态特征进行了分析。结果显示,母本东方百合‘Siberia’为二倍体即24条染色体,而父本‘Manissa’是高度杂合后代,为三倍体即36条染色体。杂交F1代的8个株系中有6个株系为二倍体即24条染色体,有2个株系为非整倍体,染色体条数分别为25和26条。母本‘Siberia’的核型为4m(1SAT)+10st(1SAT)+10t,父本‘Manis-sa’的核型为3m+18st(1SAT)+15t(1SAT),均属3B型。杂种F1代核型出现了多种类型,其中株系a、b、h为3B型,株系c、d、e、f为3A型,株系g为4B型。与亲本染色体形态相比,F1代株系出现了随体及端部着丝点染色体较多等染色体形态结构特征,而亲本没有这些特征。从染色体的形态、随体来看,子代为真杂种,在遗传上均偏向于母本。

In this study, the karyotypes of Oriental hybrids group ＇Siberia＇ ,Oriental hybrids group ＇Manis- sa＇ and their interspecific hybrids were analyzed to investigate the genetic characteristics. The results showed that female parent （＇Siberia＇） was diploid and male parent （＇Manissa＇） was triploid. 6 hybrid lines F~ were diploid,2 hybrid lines F1 were aneuploid and their number of chromosomes was 25 and 26,respec- tively. The karyotype formula of ＇ Siberia＇ was 4m （1SAT） q- 10st （1SAT） ＋ 10t belonging to ＇ 3B＇ and ＇Manissa＇ was 3m＋18st（1SAT）＋15t（1SAT） belonging to ＇3B＇ according to Stebbins classification of karyotypic asymmetry. F1 showed different karyotypes, among lines a, b and h belonged to ＇3B＇, c, d, e and f belonged to ＇3A＇, and g belonged to ＇4B＇. F1 had satellite and more teloeentrie chromosomes, so they were true progeny,with a genetic tendency to their female parent.