诱导子对百里香再生植株中苯丙氨酸解氨酶活性的影响

Effect of Elicitors on the Activity of L-Phenylalanine Transaminase in Plantlets of Thymus

以1/2MS为基本培养基,分别用蔗糖、NO3-/NH4+、苯丙氨酸、谷氨酸和酪氨酸诱导培养百里香组培苗30d,以及茉莉酸甲酯(MeJA)、水杨酸(SA)和壳聚糖诱导培养百里香20、30和40d组培苗植株0～120h,在不同的时间点测定其苯丙氨酸解氨酶(PAL)活性,以探讨诱导子对增殖百里香中PAL活性的影响。结果显示:以1/2MS为基本培养基,碳氮源调控30d后,百里香组培株的PAL活性以蔗糖浓度为2%、NO3-/NH4+比例为2∶1时最高;前体调控添加0.6mmol/L苯丙氨酸、0.8mmol/L谷氨酸和0.4mmol/L酪氨酸处理的百里香组培苗的PAL活性最高,分别为相应对照的126.3%、150.6%和153.9%。培养20d和30d植株的PAL活性均以1.0mmol/L MeJA、100mg/L SA和200mg/L壳聚糖诱导处理的最高,分别为相应对照的610.3%、788.1%、592.5%以及402.4%、541.2%、400.1%;而40d植株经0.5mmol/L MeJA、100mg/L SA和200mg/L壳聚糖诱导的PAL活性最高,分别为对照的390.2%、377.1%和413.4%。可见,在不同时期添加不同的诱导子对百里香再生植株中PAL活性有显著影响,且不同材料和诱导子存在各自适宜的诱导浓度和最佳诱导时间。

The plantlets of thyme were cultured on the 1/2 MS containing different sucrose, the NO3^-/NH4^＋, phenylalanine, glutamic acid and tyrosine,and were tested for the PAL activity. The PAl. activities were also tested after the plantlets were induced for 0-120 h by MeJA,SA and chitosan at different cul- ture times （20 d,30 d,40 d）. The results showed that the highest PAL activity was obtained when the pla- ntlets of thyme were cultured on the 1/2 MS medium with 2% sugar concentration and 2 ： 1 NO3^-/NH4^＋ ratio for 30 d. The PAL activities were increased to 126.30//oo ,150.6% and 153.9% compared with CK by adding 0.6 mmol/L phenylalanine, 0.8 mmol/L glutamic acid, and 0.4 mm% ,788.1% and 592.5% than that of CK by adding 1.0 mmol/L MeJA,100 mg/L SA,and 200 mg/L. chitosan as elicitors. The PAIr activities of plantlets cultured 30 d were increased to 402.4%, 541.2G and 400. 1% compared with CK. And the highest PAL activities of plantlets cultured 40d were increased to 390.20% ,377.1% and413.4% than that of CK. Therefore,the PAL activity was affected by adding different inducer at different culture time.