青海省小麦主栽品种遗传多样性的SSR标记分析

Analysis on Genetic Diversity among Qinghai Cultivars Wheat Based on SSR Markers

采用SSR标记对青海省66份小麦主栽品种进行遗传多样性分析,筛选出20对扩增谱带稳定的引物,共检测出81个等位基因,平均每个位点检测出的等位基因为4.05个,变异范围2～8个,引物xgwm133-6B和xgwm3-3D的等位位点最多,均为8个;其次是xgwm2-3A和xgwm107-4B,均为6个。66份小麦材料的遗传相似系数为0.530 9～0.975 3。多态信息含量PIC为0.686 5,用popgene算出Shannon’sInformation index为0.367 2,Nei’s gene diversity为0.237 6。聚类分析结果显示,66份材料聚为4大类群,部分材料在聚类时从亲缘关系上没有被明显区分,说明这些材料的生长习性与所研究的SSR引物位点相关性状存在独立性,但总体上基于SSR多态性的聚类与材料来源地区存在一定的相关性,在一定程度上反映了供试材料间的亲缘关系。

The genetic diversity among 66 wheat cultivars of Qinghai was analyzed by SSR markers.20 pairs of SSR primers with stable amplified band patterns were screened and a total of 81 alleles were scored.2 to 8 alleles could be detected by each SSR primer with the average number of 4.05.Eight alleles were detected in primer xgwm133-6B and xgwm3-3D and 6 alleles in xgwm2-3A and xgwm107-4B.The genetic similarity（GS） among 66 wheat cultivars varied from 0.530 9 to 0.975 3.The mean polymorphism information content（PIC） was 0.686 5.Shannon＇s Information index and Nei＇s（1973） gene diversity were 0.367 2 and 0.237 6 separately.Based on cluster analysis,66 wheat cultivars could be clustered into 4 groups,and some materials were not distinguished clearly by cluster analysis because of parents types,indicating that growth habits of these materials were independent with related behaviors of SSR primer loci,but clustering based on SSR polymorphism was related to ecological areas generally,which was generally agree with affiliation of experimented varieties.