牛磺酸对脑创伤大鼠脑组织代谢的影响

The Effect of Taurine on Brain Metabolism in Rats with Severe Traumatic Brain Injury

目的：研究牛磺酸对脑创伤后大鼠脑组织代谢的影响情况。方法：将30只雄性SD大鼠随机分为假手术组（sham）、脑创伤组（TBI）及牛磺酸治疗组（TAU），每组10只。TBI组和TAU组用液压打击仪制造左侧脑创伤模型，TAU组于伤后立即从尾静脉给予牛磺酸（200mg／kg）治疗，sham组和TBI组给予等量的生理盐水。用微透析探针收集各组左侧脑组织的1-3h、22～24h、7d的脑组织间液，用CMA600全自动生化分析仪检测细胞间液的葡萄糖、乳酸、甘油和丙酮酸的含量，并计算乳酸／丙酮酸的比值。结果：（1）在损伤后7d内，3组海马区脑组织的葡萄糖含量差异无统计学意义（P〉0．05）；TBI组的葡萄糖随时间的推移而显著升高。（2）TBI组的乳酸含量在3个时间点内均显著高于sham组；牛磺酸在24h后显著降低损伤后的乳酸含量，与sham组比差异无统计学意义（P〉0．05）。（3）与sham组比较，脑创伤后伤侧脑组织的甘油无显著变化，而牛磺酸在7d时显著降低其含量。（4）TBI组的乳酸／N酮酸比值在3个时间点内均显著高于sham组，并随时间延长而显著下降；牛磺酸显著降低损伤后的乳酸／丙酮酸比值，但均显著高于sham组。结论：牛磺酸对调节脑创伤后的代谢紊乱有一定的积极作用。

Objective： To observe the effect of taurine on energy metabolism in rats with traumatic brain injury. Methods： Thirty male SD rats were randomly divided into sham group, traumatic brain injury（TBI） group and taurine treatment group （TAU）, 10 rats in each group. Rats in TBI and TAU treatment group were subjected to severe traumatic injury of left brain by lateral fluid pressure device. Rats were injected traurin （200 mg/kg）in TAU treatment group and rats in other groups were given the same volume of saline through caudal vein immediately. The intercellular fluid of left brain was collected using the method of microdialysis in three groups （each group contain 3 time points,1-3 h, 22-24 h and 7 d）. The levels of glucose, lactate, glycerol and pyruvate in samples were measured using CMA600 auto biochemistry analyzer. The ratio of lactate/pyru- rate was calculated. Results： （1） There was no significant difference in the level of glucose of the left brain tissue within 7 d of traumatic brain injury between three groups（P 〉 0.05）. The level of glucose was increased significantly with time in TBT group. （2）The level of lactate was significantly higher in TBI group than that in sham group. The level of lactate significantly decreased in TAU treatment group in 24 h, which was no significant difference compared with that of sham group（P 〉 0.05）. （3） After traumatic brain injury, the level of glycerol was not significantly changed in damaged hippocampus than that of sham group, but it Was significantly decreased in TAU treatment group in 7 d.（4）The level of lactate/pyruvate was significantly higher in TBI group than that in sham group, which was significantly decreased with time. The level of lactate/pyruvate was significantly decreased in TAU treatment group, which was significantly higher than that in sham group. Conclusion： Taurine has a positive role in regulating metabolic disorders after TBI.