矽尘接触人群血清蛋白质指纹与接尘量的相关性分析

Correlation analysis between the exposure levels and the serum protein fingerprints in population exposure to silica

目的筛选矽尘接触人群血清蛋白质指纹并分析其与接尘量的相关性。方法应用液体芯片飞行时间质谱技术检测Ⅰ期矽肺组（25例）、正常对照组（30例）、矽尘接触组（30例）及可疑矽肺组（30例）血清蛋白质指纹并筛选出差异显著的蛋白，对每个样品差异蛋白的峰强度与其对应的接尘量进行相关性分析。结果4组间共筛选出5个差异显著蛋白，其中5081Da、5066Da为表达上调蛋白，3954Da、2021Da、1777Da为表达下调蛋白，将这些蛋白峰强度分别与其相应的接尘量进行相关分析，未发现蛋白峰程度与接尘量有相关关系（FI=0．039，r2=0．104，r3=-0．047，r4=-0．028，r5=-0．016，P〉0．05）。结论本次研究中矽尘接触人群血清蛋白质指纹与接尘量不存在相关关系。

Objective To explore the correlation between the exposure levels and serum protein finger- prints in population exposed to silica. Methods Liquid chip time-of-flight mass spectrometry technology was used to investigate the serum profiles in control group （30 cases）, group exposed to silica （30 cases）, silicosis group （I stage, 25 cases） and suspected silicosis group （30 cases）, and screen the differential expression proteins. The correlation between the levels of the differential expression proteins and the exposure levels was per- formed. Results Five differential expression proteins were found among 4 groups, the expression of 5081 and 5066 proteins was upregulated, and the expression of 3954, 2021 and 1777 proteins was downregulated. There was no the correlation between the exposure levels and the peak with M/Z among those proteins. Conclusion the resuhs of present investigation indicated there was no correlation between thw exposure levels and protein/ peptide peak.