萱草花提取物的指纹图谱研究

Finger Print Analysis of Orangedaylily Extract

目的:建立萱草花提取物的高效液相色谱(HPLC)指纹图谱分析方法,为有效控制萱草花提取物的质量奠定基础。方法:采用HPLC法,色谱柱为安捷伦C18柱(4.6×250mm,5μm),甲醇-0.5%醋酸水溶液梯度洗脱,检测波长为254nm,流速为1mL/min,分析时间为70min,分析了10批经大孔树脂处理的萱草花提取物的HPLC指纹图谱。结果:在选定的色谱条件下,通过相似度分析软件确定了21个色谱峰构成萱草花提取物的特征峰。结论:该分析方法稳定可靠,为萱草花提取物的质量评价与控制提供了依据。

Objective：To establish the chromatographic finger print analysis for the quality control of Orangedaylily extract. Methods ： RP - HPLC Methed was applied to establish the chromatographic finger print. The seperation was performed on a Anglent C18 column（4.6 ×250mm,5？ m） with a gradient elution composed of methanol and 0.5% acetic acid. The column temperature was set at 25℃ and the flow rate was 1 mL/min, the detective wavelength was at 254nm. Results：Under the above chromatographic conditions, the characteristic peak of finger print chromatographic of crude material of Orangedaylily extract was composed of 21 chromatographic peaks through similarity analysis. Conclusion：The finger print chromatographic set up by RP - HPLC is stable and repeatable ,which can be uesd for quality control.