36份枣品种SSR指纹图谱的构建

Construction of SSR Fingerprint on 36 Chinese Jujube Cultivars

利用12对SSR引物对36个枣品种进行分析,采用荧光M13毛细管电泳技术进行多态检测,共检测到99个多态位点,每对引物的多态位点数达到8.25,PIC值变幅为0.62~0.85,平均为0.75。依据12对SSR引物在36个品种中扩增的特异带型组合,采用引物—带型组合法构建了36个枣品种的指纹图谱,并对这36份枣品种做聚类分析,遗传相似系数在0.6667~0.9444之间。研究结果为枣树分类、种质鉴定和分子育种提供了重要的工具。

In this study,36 Chinese jujube cultivars were analyzed by capillary electrophoresis using fluorescent M13 multi-state detection method with 12 pairs of newly developed SSR primers. Totally,99 polymorphic alleles were revealed,with an average of 8.25 for each primer pairs. Polymorphism information content values for the primer pairs ranged from 0.62–0.85,with an average of 0.75. A strategy of combining primer pair with distinct alleles for fingerprint construction was developed and applied to the 36 cultivars. The genetic similarities among cultivars range from 0.6667 to 0.9444,on which a phenetic tree showing the relationship among the cultivars was constructed. The present results provide valuable tools for the cultivar classification,germplasm identification and molecular breeding of Chinese jujube.