黄瓜渐渗后代遗传变异分析及QTL检测

Analysis of Genetic Variation and QTL Detection in Cucumber Introgression Progenies

为了探讨栽培黄瓜（Cucumis sativusL.,2n=14）渐渗酸黄瓜（C.hystrix Chakr.,2n=24）遗传物质后的遗传变异情况,以12个黄瓜—酸黄瓜渐渗系及其受体亲本栽培黄瓜‘北京截头’为试验材料,对表现酸黄瓜主要特征的生物学性状进行调查分析和相关QTL检测。结果表明：各渐渗系的变异系数介于6.17%~46.26%,平均为20.81%,在多数性状上与受体亲本差异极显著,表现出酸黄瓜的遗传特性。通过t测验,以P≤0.001为阈值,在10个性状上共检测到87个QTL,平均每个性状9.67个QTL,每个渐渗系7.25个QTL,每个导入DNA片段0.8个QTL。上述结果证明控制相关性状的野生酸黄瓜遗传物质已渐渗进入栽培黄瓜,且使其遗传基础有所扩大。

To explore the genetic variations in cucumber（Cucumis sativus L.,2n = 14）—sour cucumber（C. hystrix Chakr.,2n = 24）introgression lines,twelve introgression lines and their recipient parent cultivated cucumber‘Beijing Jietou’were used in analysis of biological traits derived from sour cucumber and detection of the relevant QTLs. The results indicated that coefficient of variation among different introgression lines ranged from 6.17% to 46.26%,with an average of 20.81%. And the majority of QTL traits had significant difference between recipient parent and 12 different introgression lines, showing the genetic characters of sour cucumber. By t test to P ≤ 0.001 as the threshold value,a total of 87 QTLs were detected in 10 traits,an average of 9.67 QTLs per trait,7.25 QTLs each introgression line and 0.8 QTL each introgressed DNA segment. Above results proved that the sour cucumber genetic material,which controlled related traits,had introgressed into cultivated cucumber,and the cucumber genetic basis had been expanded.