摘要
为确定假俭草(Eremochloa ophiuroides)再生体系中种子外植体的消毒方式,经过预备试验后共设计A,B,C,D共4组2系列8种消毒方式。A1:3.5%次氯酸钠消毒25min,A2:浓硫酸灼烧30s+3.5%次氯酸钠消毒20min,B1:7%次氯酸钠消毒20min,B2:浓硫酸灼烧30s+7%次氯酸钠消毒20min,C1:0.05%升汞消毒20min,C2:浓硫酸灼烧30s+0.05%升汞消毒15min,D1:0.1%升汞消毒15min,D2:浓硫酸灼烧30s+0.1%升汞消毒10min。结果表明:A2的愈伤诱导效果最好,达到52%;D1的愈伤诱导率最低,仅17.33%,其他处理的愈伤诱导率介于二者之间。发现浓硫酸灼烧假俭草种子30s,可显著提高出愈率;试验证实消毒剂次氯酸钠比升汞有利于假俭草种子的愈伤诱导。
An efficient regeneration system is crucial to crop breeding by biotechnology.Explant sterilization methods are key factors for an efficient regeneration system.Eight sterilization methods(A1:3.5% sodium hypochlorite 25 min,A2:concentrated sulphuric acid 30 s+3.5% sodium hypochlorite 20 min,B1:7% sodium hypochlorite 20 min,B2:concentrated sulphuric acid 30 s+7% sodium hypochlorite 20 min,C1:0.05% mercury bichloride 20 min,C2:mercury bichloride 30 s+0.05% mercury bichloride 15 min,D1:0.1% mercury bichloride 15 min,D2:mercury bichloride 30 s+0.1% mercury bichloride 10 min) were tested to determine an efficient regeneration system for Centipede grass.Results showed the A2 treatment with 52% callus induction ratio as the best for Centipede grass,whereas the D1 treatment resulted in the lowest callus induction ratio(17.33%).Seeds treated by concentrated sulfuric acid with 30 s had a higher callus induction ratio than those not treated with concentrated sulfuric acid.Results also indicated that sodium hypochlorite was more efficient than mercury bichloride for Centipede grass callus induction.
出处
《草地学报》
CAS
CSCD
北大核心
2012年第2期383-388,共6页
Acta Agrestia Sinica
基金
广东省科技计划项目(2006B20201035)
湖南农业大学校青年科学基金(2010QN30)资助
关键词
假俭草
消毒方式
愈伤诱导
Centipedegrass
Sterilization methods
Callus induction