影响血浆化浆效果的因素分析

Slurry Time and Temperature on The Effect of Pulp

目的:血浆解冻过程中,有时会出现少量的絮状沉淀物,血浆纤维蛋白析出,不能输用,造成了血浆的浪费,所以如何提高化浆效果,使得絮状沉淀物完全溶解,成为了急待解决的问题。本文探讨了冰冻血浆融化后出现絮状物的原因及解决办法。方法:冰冻血浆置37℃循环解冻水浴箱迅速融化,也可用37℃与41℃数码恒温循环解冻箱(干式)溶浆,在有纤维蛋白析出血浆袋中也可两种仪器配合使用,或用血小板保存箱振摇化浆,再将几种方法化浆效果进行对比分析。结果:用循环解冻水浴箱解冻,循环水流,快速加热,出现絮状沉淀物大大减少,一般情况下15 min到25 min即可将血浆完全溶解。数码恒温循环解冻箱(干式)解决了污染与血袋标识模糊问题,但化浆速度较慢,数码恒温循环解冻箱(干式)41℃水浴37 min化浆效果最理想。结论:掌握溶浆温度与时间是提高化浆效果的关键。较高的温度虽然可加速血浆的融化,但会加速浆中蛋白质的变性,较高的温度或温度过低均可造成凝血因子不同程度的损耗。先将冰冻血浆置于37℃恒温循环解冻水浴箱,如仍有血浆纤维蛋白析出,应用37℃与41℃数码恒温循环解冻箱(干式)振动模式化浆十几min可明显提高溶浆效果。融化方法不当,储存不当,采血不顺利,血浆制作过程中时间与温度未掌握好等均会影响化浆效果。对于较难溶解的血浆袋可用干法与湿法化浆配合使用,但要保障溶浆时间不能过长。

Objective： The plasma thawing process, sometimes a small amount of flocculent precipitate, precipitation of plasma fibrinogen, can not lose with, resulting in a plasma waste, so how to improve the effect of pulp, making the floeeulent precipitate is completely dissolved, a pressing problem. This paper discusses the melting of frozen plasma after floc causes and solutions. Methods： Frozen plasma thawing cycles at 37℃ water bath to melt rapidly, can also be 37℃ and 41℃ digital thermostat box thaw cycle （dry type） soluble, fibrin precipitation in a plasma bag can also be used in conjunction with the two instruments, or platelet preservation box with shaking of the pulp, and then several methods of plasma effects were analyzed. Results： The circulating water bath thaw thaw cycle of water, rapid heating, flocculent precipitate appeared greatly reduced, generally 15 minutes-25 minutes to completely dissolve the plasma. Digital thermostat box thaw cycle （dry） to solve the pollution problem with the blood bag identification fuzzy, but slurry slow thaw cycle digital thermostat box （dry） 41℃water bath for 37 minutes slurry best results. Conclusions： The temperature and time to master dissolving pulp slurry is to improve the effectiveness of the key. Although a higher temperature can accelerate the melting of the plasma, but will accelerate the plasma protein denaturation, high temperature or the temperature is too low can cause varying degrees of loss of coagulation factors. First frozen plasma thawed at 37℃ constant temperature circulating water bath, as there is still plasma fibrinogen precipitation, application of 37℃ and 41℃thaw cycle of the digital thermostat box （dry） vibration mode of the plasma ten minutes can significantly increase the effect of dissolving pulp. Melting method properly, stored properly, blood does not go smoothly, the plasma production process does not have a good time and temperature etc will affect the results of pulp. For the dissolution of the plasma bags can be difficult to dry and wet slurry with, but to protect the soluble time not too long.