电磁场对果蝇唾液腺细胞蛋白质组影响的研究

Study for the Effects of Electrical Magnetic Exposure on Salivary Glands Proteome of Drosophila Melanogaster

目的:研究50 Hz工频电磁场对果蝇唾液腺细胞蛋白质表达谱的影响。方法:将野生黑腹果蝇置于0.5 mT工频电磁场中30 min,取唾液腺蛋白质,进行双向凝胶电泳,分析磁场处理组与未经辐射的果蝇唾液腺蛋白质表达差异。结果:对照组3次凝胶的平均蛋白质点数为514,电磁场处理组的3次凝胶的平均蛋白质点数为462。选取所有胶上相互匹配且分辨清楚的蛋白质点进行统计学分析,筛选并鉴定出具有明显差异(Varition>2.0,P<0.05)的蛋白质点9个。磁场处理后,果蝇唾液腺组织中的异柠檬酸脱氢酶,Vacuolar ATP synthase、果糖二磷酸醛缩酶、肌动蛋白去聚合因子、延伸因子1-α、延伸因子1-β、40S核糖体蛋白、磷酸甘油酸激酶、蛋白质二硫键异构酶表达均升高。结论:电磁场对于果蝇唾液腺作用后,并未产生热休克蛋白,其作用机制区别于热休克反应。电磁场改变果蝇唾液腺细胞膜的通透性与细胞内环境,以此为起点调控细胞内的信号传导,使细胞能量代谢加快,敏感蛋白表达增加,细胞骨架改变。

Objective： To study the proteomic difference of drosophila salivary gland cells in 50 Hz electromagnetic fields. Methods：We put drosophila melanogaster in 0.5 mT electromagnetic fields for 30 min,then extracted the intact protein in the salivary for 2DE. The gels were scanned and analyzed by ImageMaster6.0 software,and the different proteins were identified by mass spectrum. Results： The average spots in control group were 514,while treated group were 462 after matching. 9 proteins were identified,the expression of isocitrate dehydrogenase,Vacuolar ATP synthase,ffuctose-bisphosphate aldolase, actin depolymerizing factor,elongation factorl-α and 1-β,40S ribosomal protein,phosphoglyceric kinase and protein disulfide isomerase increased after electrical magnetic exposure. Conclusions： Heat shock proteins were not found in the treated group, which means the effect of electrical magnetic exposure was not the same with Heat-Shock Response.Moreover, electromagnetic fields might change cell membrane permeability and internal environment,which contribuated to transduction of intracellular signal pathway, accelerated energy metabolism,increased susceptible proteins,and changed cell skeleton.