原发性干燥综合征患者外周血中IL-27水平降低及其意义

Low level of plasma interleukin 27 in patients with primary Sjgren syndrome

目的探讨IL-27在原发性干燥综合征患者外周血中的表达情况及其与疾病的相关性。方法收集初诊原发性干燥综合征患者与同期健康体检者各31例,采用RT-PCR、ELISA等方法检测其外周血中IL-27的表达情况,并分析其与IgG、IgE、抗SSA(Ro)抗体、抗SSB(La)抗体、ESR水平及CRP等指标的相关性。结果原发性干燥综合征患者外周血单个核细胞中IL-27/P28及IL-27/EBI3mRNA相对表达量及其血浆IL-27浓度均显著低于健康对照组;原发性干燥综合征患者血浆IL-27浓度与抗SSA抗体、抗SSB抗体及IgE水平存在一定相关性。结论IL-27在原发性干燥综合征患者外周血中表达降低,且与原发性干燥综合征的发生发展存在一定的相关性,其可能参与了原发性干燥综合征的发病过程。

To detect the serum interleukin 27（IL-27） level in primary Sjogren syndrome（pSS） patients and its relations to char- acteristic parameters of the disease, 31 patients with pSS and 31 healthy volunteers were recruited. The expression of IL-27 in peripheral blood mononuclear cells（PBMCs） and plasma were detected by RT-PCR and ELISA, respectively. The disease labo- ratory parameters were collected from medical records. The plasma IL-27 level and IL-27 mRNA relative expression in PBMCs of pSS patients were significantly lower than those in healthy controls （P〈0.05）. The plasma IL-27 level was negatively corre- lated with serum anti-SSA antibody, serum anti-SSB antibody and IgE level, but not with serum IgG and CRP concentration, as well as ESR level. The low level of plasma IL-27 in pSS patients and the relationship with disease characteristic parameters suggested that this cytokine might be implicated in the pathomechanism of pSS.