HMGB1在肾透明细胞癌中的表达及其临床意义

The expression of HMGB1 in human renal clear cell carcinoma and its clinical significance

目的研究高迁移率族蛋白B1(HMGB1)在肾透明细胞癌(renal clear cell carcinoma,RCCC)中的表达及临床意义。方法收集40例肾透明细胞癌、70例癌旁肾组织、20例正常肾组织标本,采用免疫组织化学二步法检测HMGB1的表达,并分析其与临床病理参数之间的关系。结果 HMGB1在正常肾组织、癌旁组织、RCCC中的阳性表达率逐渐升高(P<0.05);RCCC中,HMGB1阳性表达率在临床Ⅲ期、Ⅳ期高于Ⅰ期、Ⅱ期,有淋巴结转移组高于无淋巴结转移组,肿瘤直径≥5cm组高于肿瘤直径<5cm组,肿瘤坏死组高于无坏死组(均P<0.05);其表达与年龄、性别、分化程度、肿瘤所在部位均无关(P>0.05)。结论 HMGB1蛋白的表达参与了RCCC的发生、发展,可以作为判断RCCC预后的分子指标。

Objective To investigate the expression and its clinical significance of HMGB1 in human renal clear cell carcinoma（RCCC）.Methods The expression of HMGB1 in 70 cases of RCCC,40 cases of normal renal tissues adjacent neoplasm and 20 cases of normal renal tissues were detected by immunohistochemically and the correlation between the expression and clinicopathological parameters were analyzed.Results The positive expression rates of HMGB1 in RCCC tissues,normal renal tissues adjacent neoplasm,normal renal tissues were 25.0%,37.5%,82.8% respectively,the trend had significantly increased （P0.05）.In RCCC,the positive expression rate of HMGB1 in clinical stages Ⅲ-Ⅳ group,lymph node metastasis group,tumor diameter over 5cm and necrosis group were significantly higher than the Ⅰ-Ⅱ group,no lymph node metastasis group,tumor diameter lower than 5 cm and no necrosis group,while the positive expression rate of HMGB1 had no relationship with age,sex,position and the degree of tumor differentiation（P0.05）.Conclusions The high expression of HMGB1 may be involved in the progression of RCCC.HMGB1 can be used as a useful molecular marker for prognosis of RCCC.