期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

BATF2对Wnt信号通路影响的研究

The Investigation of Influence on Wnt Signaling Pathway by BATF2
原文传递
导出
摘要 Wnt信号通路在各种生物体内高度进化保守,与癌症的发生发展密切相关。BATF2是一个新近发现的基因,研究表明其具有抑癌基因的作用。目前,BATF2与Wnt信号通路的关系尚不清楚,该文用荧光素酶报告基因检测、Real-time PCR和Western blot发现BATF2能影响Wnt信号通路。过表达BATF2可明显下调TCF4/β-catenin的转录活性和Wnt信号通路下游基因的表达,可以下调细胞核中的β-catenin。推测BATF2可能通过下调细胞核中的β-catenin来实现对Wnt信号通路的下调。上述结果为抑制Wnt信号通路用于肿瘤治疗提供了一定的依据。 Abstract Wnt signal pathway is highly conserved in different species throughout evolution, which plays a crucial role in tumor genesis and progression. BATF2 is a novel gene, which has shown the antitumor activity. But the relationship between Wnt pathway and BATF2 has not been clearly investigated. In this study, we revealed that BATF2 could influence the Wnt signaling pathway by luciferase report assay, Real-time PCR and Western blot. The overexpression of BATF2 could significantly reduce the TCF4/β-catenin transcriptional activity and the Wnt target genes expression, and downregulate the β-catenin in the nuclear. We hypothesized that the BATF2 reduced the Wnt signaling pathway through downregulating β-catenin in nuclear. The results provided an antitumor therapy through inhibit Wnt signaling pathway.
作者 郑水娣 吴帅 桂镜华 贾晓渊 章康健
机构地区 浙江理工大学生命科学学院新元医学与生物技术研究所 中国科学院上海生命科学研究院生物化学与细胞生物学研究所
出处 《中国细胞生物学学报》 CAS CSCD 北大核心 2012年第4期343-348,共6页 Chinese Journal of Cell Biology
基金 浙江理工大学研究生创新基金(No.YCX-S11021) 浙江理工大学科研启动基金(No.1016834-Y No.1016845-Y) 浙江省自然科学基金(No.Y2090935)资助项目~~
关键词 BATF2 WNT信号通路 TCF4/β-catenin转录活性 Β-CATENIN BATF2 TCF4/β-catenin transcriptional activity Wnt signaling pathway β-catenin
分类号 R730.5 [医药卫生—肿瘤]
  • 相关文献

参考文献27

  • 1MacDonald BT,Tamai K,He X.Wnt/beta-catenin signaling:Components,mechanisms,and diseases.Dev Cell 2009;17(1):9-26.
  • 2Angers S,Moon RT.Proximal events in Wnt signal transduction.Nat Rev Mol Cell Biol 2009;10(7):468-77.
  • 3Shtutman M,Zhurinsky J,Simcha I,Albanese C,D’Amico M,Pestell R,et al.The cyclin D1 gene is a target of the beta-catenin/LEF-1 pathway.Proc Natl Acad Sci USA 1999;96(10):5522-7.
  • 4Polakis P.Wnt signaling and cancer.Genes Dev 2000;14(15):1837-51.
  • 5Klaus A,Birchmeier W.Wnt signaling and its impact on devel-opment and cancer.Nat Rev Cancer 2008;8(5):387-98.
  • 6Logan CY,Nusse R.The Wnt signaling pathway in developmentand disease.Annu Rev Cell Dev Biol 2004;20:781-810.
  • 7Gattinoni L,Zhong XS,Palmer DC,Ji Y,Hinrichs CS,Yu Z,etal.Wnt signaling arrests effector T cell differentiation and gener-ates CD8+memory stem cells.Nat Med 2009;15(7):808-13.
  • 8Leow PC,Tian Q,Ong ZY,Yang Z,Ee PL.Antitumor activityof natural compounds,curcumin and PKF118-310,as Wnt/beta-catenin antagonists against human osteosarcoma cells.InvestNew Drugs 2010;28(6):766-82.
  • 9Su ZZ,Lee SG,Emdad L,Lebdeva IV,Gupta P,Valerie K,et al.Cloning and characterization of SARI(suppressor of AP-1,regu-lated by IFN).Proc Natl Acad Sci USA 2008;105(52):20906-11.
  • 10Dorsey MJ,Tae HJ,Sollenberger KG,Mascarenhas NT,Johans-en LM,Taparowsky EJ.B-ATF:A novel human bZIP protein thatassociates with members of the AP-1 transcription factor family.Oncogene 1995;11(11):2255-65.

二级参考文献26

  • 1Zhang X, Miao X, Guo Y, et al. Genetic polymorphisms in cell cycle regulatory genes MDM2 and TP53 are associated with susceptibility to lung cancer [ J ]. Hum Mutat, 2006, 27 ( 1 ) : 110-117.
  • 2Hassler M, Singh S, Yue W W, et al. Crystal structure of the retinoblastoma protein N domain provides insight into tumor suppression, ligand interaction, and holoprotein architecture [J]. MolCell, 2007, 28(3): 371-385.
  • 3Beg A A, Baltimore D. An essential role for NF-kappaB in preventing TNF-alpha-induced cell death [ J]. Science, 1996, 274(5288) : 782-784.
  • 4Hayden M S, Ghosh S. Signaling to NF-kappaB [ J]. Genes Dev, 2004, 18(18) : 2195-2224.
  • 5Liu Z G, Hsu H, Goeddel D V, et al. Dissection of TNF receptor 1 effector functions: JNK activation is not linked to apoptosis while NF-kappaB activation prevents cell death [ J ]. Cell, 1996, 87(3) : 565-576.
  • 6Ghosh S, Karin M. Missing pieces in the NF-kappaB puzzle [J]. Cell, 2002, 109(Suppl) : $81-$96.
  • 7Karin M, Greten F R. NF-kappaB: linking inflammation and immunity to cancer development and progression [ J]. Nat Rev Immunol, 2005, 5( 10): 749-759.
  • 8Shishodia S, Aggarwal B B. Nuclear factor-kappaB : a friend or a foe in cancer? [J]. Biochem Pharmacol, 2004, 68(6) : 1071- 1080.
  • 9Meylan E, Dooley A L, Feldser D M, et al. Requirement for NF-kappaB signalling in a mouse model of lung adenocarcinoma [J]. Nature, 2009, 462(7269): 104-107.
  • 10Huang W C, Ju T K, Hung M C, et al. Phosphorylation of CBP by IKKalpha promotes cell growth by switching the binding preference of CBP from p53 to NF-kappaB [ J]. Mol Cell, 2007, 26(1) : 75-87.

共引文献13

中国细胞生物学学报
2012年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部