婴儿双歧杆菌的耐氧驯化及应用特性研究

Studies on oxygen tolerance test and application characteristics of Bifidobacterium infantis

研究婴儿双歧杆菌的生长促进剂,并对活化菌种进行耐氧驯化培养,探讨婴儿双歧杆菌在搅拌花生酸奶加工中的应用。结果表明:酵母浸出液对婴儿双歧杆菌活化影响最大,酪蛋白胨次之,磷酸氢二钾最小,三者最佳水平组合:酵母浸出液0.4%,酪蛋白胨0.2%,磷酸氢二钾0.1%。菌种被连续活化三代后凝乳时间稳定至3.5h。通过对4种因素婴儿双歧杆菌生长的影响研究发现:鲜牛奶与花生乳配比的影响最大,其次是蔗糖,再次是接种量,最后是葡萄糖。当鲜牛奶与花生乳配比1∶1,蔗糖6%,葡萄糖2%,接种量5%,婴儿双歧杆菌达到最佳生长状态,活菌数达到2.5×109 CFU/mL。

This paper screened specifically Bifidobacterium infantis,and researched the activation method of inoculating culture on oxygen resistance strains,groped for the strain the application in mixing of peanut yoghourt.Results indicated that the yeast leaching solution for the strain activation had the biggest influence,and then in proper order were respectively casein peptone,dipotassium phosphate,and three best combinations were obtained that yeast leaching solution 0.4% casein peptone 0.2%,dipotassium phosphate 0.1%.The milk coagulation time stability to 3.5 h after the strains was continuously activation three generations.Through the four factors on the influence growth of Bifidobacterium infantis found： ratio of milk and peanut-milk existed the biggest influence,followed by sucrose,once again,was the inoculation amount,and finally the glucose.When milk and peanut-milk ratio 1∶1,sucrose 2%,glucose 6%,inoculation quantity 5%,Bifidobacterium infantis got to achieve the best growth state,living bacterium number reached 2.5 ×109 CFU/mL.