电针督脉和膀胱经对实验性抑郁症大鼠行为学及海马NSE表达的影响

Effects of Du Meridian and Foot Taiyang Meridian Electro-acupuncture on Behavior Changes and Expression of NSE on Hippocampal in Depression Rats

目的:观察电针督脉经穴(百会、神庭)加膀胱经穴(心俞、肝俞)对实验性抑郁症大鼠行为学和海马NSE阳性细胞表达数量的影响。方法:采用慢性应激结合孤笼饲养制作大鼠抑郁症模型,用旷场法进行行为学测定以及计算大鼠糖水消耗量,并运用免疫荧光双标技术观察对抑郁症大鼠海马NSE阳性细胞表达数量的影响。结果:电针督脉加膀胱经穴可显著改善抑郁症大鼠的体重增加迟缓,糖水消耗增多,行为学评分升高,使抑郁症大鼠海马CA1、CA3区及齿状回(DG)神经元数目显著增加。结论:针刺督脉加膀胱经穴可以使抑郁大鼠活动增多、快感增加、体重增加,减轻海马神经元的损伤,从而促进大鼠抑郁的改善。

Objective： To study the effects of Du meridian and foot Taiyang meridian eletro-acupuncture on behavior changes and expression of NSE on hippocampal in the treatment of depression.Methods：The depression models of rats were established by chronic unpredictable mild stress combining raising seperately.The rearing score and crossing score of rats were measured by open-field test and sweet water wastage was detected and the numbers of NSE positive cells were observed by double-signed immunofluorescence.Results：Du meridian and foot Taiyang meridian eletro-acupuncture could put on the pounds and increase sweet water wastage,improve the locomotor behavior of the depression rats and increase the numbers of nerve cells in CA1,CA3 and dentate gyrus on hippocampal.Conclusion：Du meridian and foot Taiyang meridian eletro-acupuncture can improve the depression rats＇ activity,pleasant feeling,body weight and protect nervel cells in hippocampal to treat depression.