葛根素注射液治疗糖尿病性骨质疏松的实验研究

Experiment Study on Protection of Puerarin in Diabetic Osteoporosis

目的:探讨葛根素注射液对糖尿病骨质疏松的保护作用。方法:30只SD大鼠随机分为正常对照组、糖尿病组和糖尿病葛根素治疗组(n=10)。糖尿病组和糖尿病葛根素治疗组大鼠经腹腔注射链脲佐菌素(65mg/kg)制作糖尿病模型,成模后糖尿病葛根素治疗组每天腹腔注射葛根素注射液80mg/kg,正常对照组和糖尿病组注射等量生理盐水。成模6周后麻处死大鼠,分离各组大鼠的左侧股骨,于小动物放射成像系统上,测其近段骨密度,HE染色进行形态学观察。结果:成模6周后,糖尿病组骨密度显著低于对照组,糖尿病大鼠其股骨头表面成骨细胞与对照组比较显著减少,空骨陷窝明显增多,而葛根素治疗组上述指标均明显改善。结论:高糖可影响骨组织代谢及成骨细胞的增殖分化,造成糖尿病骨质疏松症,而葛根素对骨质疏松的进程可起到一定的保护作用。

Objective：To explore the protective effect of puerarin on diabetic osteoporosis rats.Methods：Thirty male SD rats were divided into three groups,including control group（intraperitoneally injected saline 6 mL/kg）,diabetic group（intraperitoneally injected STZ 65 mg/kg）and puerarin treatment group（intraperitoneally injected STZ 65 mg/kg,and intraperitoneally injected puerarin 80 mg/kg/day for 6 weeks）.After 6 weeks,the rats were isolated left femur,the bone mineral density of proximal femur was measured by the small animal x-ray imaging device,and the morphological changes were also observed.Results：Bone mineral density in diabetic group was significantly lower than that in control group.Haematoxylin-eosin（HE）staining of femur revealed the femoral head surface of osteoblasts was a significant reduce and empty lacunae increased.But the osteoporosis performance was significantly improved in the puerarin group rats.Conclusion：High blood sugar can affect bone metabolism and osteoblasts proliferation and differentiation,resulting in diabetes,and the puerarin may play a protective role in the process.