梓树属植物基因组DNA提取及RAPD体系优化

Genomic DNA Extraction and Optimization of RAPD System of Catalpa Scop

以楸树幼嫩叶片为材料,进行楸树基因组DNA提取及RAPD扩增条件优化,试验表明,采用改良的2×CTAB法提取的DNA质量较高,适宜于RAPD分析。RAPD反应的优化体系为(25 ul反应体系):17.8 ul ddH2O;0.5 ul dNTP;2.5 ul 10×buffer;1.0 ul随机引物;2.0 ul Mgcl2;1.0 ul模版;0.2 ul Taq酶。

The method of genomic DNA extraction from new leaves and the optimization of RAPD analytic conditions were studies in Catalpa bungei. The result showed that the high-quality genomic DNA wasobtained by the revised 2 × CTAB method. The optimal PCR system for RAPD analysis was as foUows：17.6ul ddH2O;0.5ul dNTP;2.5ul 10× buffer; 1.01ul random Primer;2.0ul Mgcl2;1.0ul template;0.4ul Taq polymerase in 25ul reaction system.