作物青枯病植物疫苗工程菌FJAT-T8发酵过程生长适合度研究

Growth Fitness in Fermentation Process of FJAT-T8,an Avirulent Ralstonia Aolanacearum Used as Plant Vaccine Against Crop Bacterial wilt Disease

采用50L全自动发酵罐进行植物疫苗工程菌FJAT-T8发酵,并对发酵过程的相关参数进行跟踪检测。为了研究FJAT-T8的生长适合度,将发酵过程中变化差异显著的参数作为指标进行聚类分析;同时,用多元线性回归及相关法分析各参数动态模型及因子间关系,并用电镜扫描法跟踪菌体形态变化。研究结果表明:菌体生长可划分为4个周期:0～16h(适应期)、24h(对数期)、32～72h(稳定期)和80～96h(消亡期);该菌发酵最佳质量的终止判断指标为:发酵时间40～48h,发酵液pH值8.7～8.8,溶氧量趋于稳定不变。

The paper dealt with the fermentation technique of an avirulent Ralstonia solanacearum strain FJAT-T8,which was used as plant vaccine against crop bacterial wilt disease.The fermentation process of FJAT-T8 was investigated by determination of on-line and off-line parameters in 50L automatic fermentation container.In order to study the growth fitness of FJAT-T8,the parameters with significantly variation during fermentation process were served as indexes and analyzed with cluster analysis.The multiple linear regression and correlation analysis were also used to analysis the parameter dynamic model and relationship between fermentation factors.Besides,tracing observation of cell morphology was conducted with electron microscope.The results showed that the cell growth cycle of FJAT-T8 could be divided into four periods： 0-16h（adaptation period）,24h（logarithmic period）,32-72h（stationary period） and 80-96h（decline period）.The comprehensive evaluation indexes for the best fermentation quality were： fermentation time of 40-48h,fermentation pH of 8.7-8.8 and the dissolved oxygen maintaining steady.The study would improve the fermentation quality,formation of product quality standards and factory production of plant vaccine strain T8.